...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrier.
【24h】

Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrier.

机译：氧-葡萄糖的剥夺增加了组成血脑屏障的细胞的酶活性和微囊泡介导的胞外核苷酸释放。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The blood-brain barrier (BBB), the dynamic interface between the nervous tissue and the blood, is composed by endothelial cells, pericytes and astrocytes. Extracellular nucleotides and nucleosides and their receptors (the purinergic system) constitute a widely diffused signaling system involved in many pathophysiological processes. However, the role of this system in controlling BBB functions is still largely unknown. By using cultures of these three cell types grown separately and a BBB in vitro model consisting of triple co-cultures, we studied for the first time the expression and distribution of the ecto-enzymes nucleoside triphosphate diphosphohydrolases (NTPDases, the enzymes which hydrolyze extracellular nucleotides) under control and ischemic (oxygen-glucose deprivation in vitro; OGD) conditions. NTPDase1 was detected in all three cell types, whereas NTPDase2 was expressed by astrocytes and pericytes and, to a lesser extent, by endothelial cells. Endothelial cells were extremely susceptible to cell death when OGD was applied to mimic in vitro the cytotoxicity induced by ischemia, whereas astrocytes and pericytes were more resistant. A semi-quantitative assay highlighted markedly increased e-ATPase activity following exposure to OGD in all three cell types, either when grown separately or when co-cultured together to resemble the composition of the BBB. Moreover, electron microscopy analysis showed that both endothelial cells and astrocytes shed microvesicles containing NTPDases from their membrane, which may suggest a novel mechanism to increase the breakdown of ATP released to toxic levels by damaged BBB cells. We hypothesize that this phenomenon could have a protective and/or modulatory effect for brain parenchymal cells. This in vitro model is therefore useful to study the role of extracellular nucleotides in modulating BBB responses to ischemic events, and to develop new effective purinergic-based approaches for brain ischemia.
机译：血脑屏障（BBB）是神经组织和血液之间的动态界面，由内皮细胞，周细胞和星形胶质细胞组成。细胞外核苷酸和核苷及其受体（嘌呤能系统）构成了广泛传播的信号系统，涉及许多病理生理过程。但是，该系统在控制BBB功能中的作用仍然未知。通过使用分别生长的这三种细胞类型的培养物和由三重共培养物组成的BBB体外模型，我们首次研究了外切酶核苷三磷酸二磷酸水解酶（NTPDases，水解细胞外核苷酸的酶）的表达和分布。 ）处于控制和局部缺血（体外氧葡萄糖剥夺; OGD）条件下。在所有三种细胞类型中均检测到NTPDase1，而NTPDase2在星形胶质细胞和周细胞中表达，在较小程度上通过内皮细胞表达。当将OGD应用于体外模拟缺血诱导的细胞毒性时，内皮细胞极易受到细胞死亡的影响，而星形胶质细胞和周细胞的耐药性更高。半定量分析突出显示了三种细胞类型中暴露于OGD之后的e-ATPase活性显着增加，分别生长或共同培养以类似于BBB的成分时。此外，电子显微镜分析表明，内皮细胞和星形胶质细胞都从其膜上脱落了含有NTPDase的微囊泡，这可能暗示了一种新的机制，可将受损的BBB细胞释放的ATP分解增​​加至毒性水平。我们假设这种现象可能对脑实质细胞具有保护和/或调节作用。因此，该体外模型可用于研究细胞外核苷酸在调节BBB对缺血事件的反应中的作用，以及开发新的基于嘌呤能的新型有效方法来治疗脑缺血。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号