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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Increased expression of glyceraldehyde-3-phosphate dehydrogenase in cultured astrocytes following exposure to manganese.
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Increased expression of glyceraldehyde-3-phosphate dehydrogenase in cultured astrocytes following exposure to manganese.

机译：暴露于锰后培养的星形胶质细胞中甘油醛-3-磷酸脱氢酶的表达增加。

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摘要

Manganism is a disorder characterized by hyperintensities in basal ganglia structures on magnetic resonance imaging which may be the consequence of manganese deposition in these areas. Since manganese is taken up avidly into astrocytes and is known to interfere with cerebral energy metabolism, we studied the effect of this metal on the expression and activity of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in primary cultures of astrocytes. Treatment with 100 microM manganese for 7 days increased both the Vmax and Km values for GAPDH which was not reproducible with other divalent metals. Using RT-PCR, increased GAPDH expression was detected in cells exposed to manganese compared with controls. No changes in cytochrome oxidase activity or ATP levels were observed, and lactate production was unaffected, in manganese-treated cells. These findings provide evidence of a possible role for GAPDH in the mediation of the effects of manganese on central nervous system function.
机译：锰病是一种以磁共振成像为基础的神经节结构过度增高为特征的疾病，这可能是这些区域中锰沉积的结果。由于锰被大量摄取到星形胶质细胞中，并且已知会干扰脑能量代谢，因此我们研究了这种金属对星形胶质细胞原代培养物中糖酵解甘油三磷酸甘油醛脱氢酶（GAPDH）的表达和活性的影响。用100 microM锰处理7天增加了GAPDH的Vmax和Km值，这是其他二价金属无法再现的。使用RT-PCR，与对照相比，在暴露于锰的细胞中检测到GAPDH表达增加。在锰处理过的细胞中，未观察到细胞色素氧化酶活性或ATP水平的变化，并且乳酸的产生未受影响。这些发现提供了GAPDH在调节锰对中枢神经系统功能的影响中可能发挥作用的证据。

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