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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.
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Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.

机译：高钾增强脂多糖诱导的星形胶质细胞产生一氧化氮的产生。

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摘要

Uptake of K+ is an important role of astrocytes to maintain physiological lower extracellular K+ concentration in the CNS. In this study, the effect of high K+ concentration was examined on the cellular function of astrocytes from embryonic rat brain in primary culture. Nitric oxide (NO) production induced by lipopolysaccharide (LPS) was measured as an index of cellular function of astrocytes. Increasing KCl concentration to about 40 mM did not directly evoke NO production, but doubled the level of LPS (1 ng/ml)-induced NO production. K-gluconate showed a similar enhancing effect although the degree of enhancement was about half of that of KCl. Neither NaCl nor Na-gluconate showed any effect. The K(+)-channel blocker, 4-aminopyridine, but not tetraethylammonium or apamin, inhibited the enhancing effect of KCl. The LPS-induced iNOS protein expression determined by immunoblotting analysis was enhanced by high K+ treatment. The level of iNOS mRNA determined by real-time RT-PCR technique was also augmentedby the presence of 40 mM KCl. These results indicate that the elevation of extracellular K+ concentration regulates astrocytic cell functions through a mechanism involving K(A)-type K(+)-channels and that potentiation of NO production by high K+ is due to the augmentation of iNOS mRNA and iNOS protein levels.
机译：摄取K +是星形胶质细胞在中枢神经系统中维持生理较低的细胞外K +浓度的重要作用。在这项研究中，检查了高K +浓度对原代培养中胚胎大鼠脑星形胶质细胞功能的影响。测量由脂多糖（LPS）诱导的一氧化氮（NO）生成，作为星形胶质细胞的细胞功能指标。将KCl浓度增加到约40 mM并不能直接引起NO产生，但会使LPS（1 ng / ml）诱导的NO产生水平增加一倍。葡萄糖酸钾显示出相似的增强作用，尽管增强程度约为氯化钾的一半。 NaCl和葡萄糖酸钠均未显示任何作用。 K（+）通道阻滞剂，4-氨基吡啶，但不是四乙铵或木瓜蛋白酶，抑制了KCl的增强作用。通过免疫印迹分析确定的LPS诱导的iNOS蛋白表达通过高K +处理得以增强。通过实时RT-PCR技术测定的iNOS mRNA水平也因存在40 mM KCl而增加。这些结果表明，细胞外K +浓度的升高通过涉及K（A）型K（+）-通道的机制调节星形胶质细胞功能，而高K +产生NO的增强是由于iNOS mRNA和iNOS蛋白的增加水平。

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