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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >The importance of synapsin I and II for neurotransmitter levels and vesicular storage in cholinergic, glutamatergic and GABAergic nerve terminals.
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The importance of synapsin I and II for neurotransmitter levels and vesicular storage in cholinergic, glutamatergic and GABAergic nerve terminals.

机译：突触素I和II对胆碱能，谷氨酸能和GABA能神经末梢神经递质水平和囊泡贮藏的重要性。

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The aim of this study was to examine the importance of the vesicle-associated synapsin I and II phosphoproteins for the accumulation of neurotransmitters in central cholinergic as compared to central glutamatergic and GABAergic nerve terminals. In brain homogenate samples from mice devoid of synapsin I and II, the levels of vesicular transporters for glutamate (VGLUT1-2) and GABA (VGAT) were decreased by 35-40% in striatum and cortex, while no change was apparent for the vesicular acetylcholine transporter (VAChT). The severe decrease in the levels of amino acid vesicular transporters caused only minor changes in the concentrations of the respective neurotransmitters in homogenates of the three selected brain areas from synapsin I- and II-deficient mice. However, when measured in a crude vesicular fraction, the concentrations of glutamate and GABA were decreased by 48-60% in synapsin-deficient mice, with a similar decrease in the levels of VGLUT1, VGLUT2 and VGAT. In comparison, the concentration of acetylcholine and the level of VAChT were not significantly different from wild-type in the vesicular fraction. No changes were seen in the activity of specific enzymes involved in the synthesis of acetylcholine, glutamate or GABA, however, immunoblotting indicated a decrease in the protein level of glutamic acid decarboxylase, isoform 65 (GAD(65)). In conclusion, the results indicate that neurotransmitter regulation in central cholinergic synapses may be less dependent on synapsin I and II compared to the marked alterations seen in the glutamatergic and GABAergic synapses.
机译：这项研究的目的是检查与中央谷氨酸能和GABA能神经末梢相比，囊泡相关突触蛋白I和II磷酸蛋白对于中枢胆碱能神经递质蓄积的重要性。在没有突触素I和II的小鼠的脑匀浆样品中，纹状体和皮层中谷氨酸（VGLUT1-2）和GABA（VGAT）的囊泡转运蛋白水平降低了35-40％，而囊泡没有明显变化乙酰胆碱转运蛋白（VAChT）。氨基酸囊泡转运蛋白水平的严重降低仅导致突触素I和II缺陷小鼠的三个选定大脑区域匀浆中相应神经递质的浓度仅发生微小变化。但是，当在粗小囊泡级分中测量时，突触蛋白缺乏小鼠的谷氨酸和GABA浓度降低了48-60％，VGLUT1，VGLUT2和VGAT的水平也有类似的降低。相比之下，水泡部分的乙酰胆碱浓度和VAChT水平与野生型无显着差异。没有观察到参与乙酰胆碱，谷氨酸或GABA合成的特定酶的活性发生变化，但是，免疫印迹表明谷氨酸脱羧酶同工型65（GAD（65））的蛋白质水平降低了。总之，结果表明，与在谷氨酸能和GABA能突触中观察到的显着变化相比，中枢胆碱能突触的神经递质调节可能较少依赖突触蛋白I和II。

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