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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Metabolic and cellular characterization of immortalized human microglial cells under heat stress.
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Metabolic and cellular characterization of immortalized human microglial cells under heat stress.

机译：在热应激下永生化的人类小胶质细胞的代谢和细胞特征。

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Microglia are able to shift from a resting to an activated state during which they acquire functions typical of primitive forms of mononuclear phagocytes, as well as highly differentiated forms of these cell types. Activation of the microglia after a trauma involves functional readjustments including both the resurrection of basic cell functions and the development of functions implicated in cell-cell interactions. Here, the behaviour of the human microglial cell line CHME-5 was observed after hyperthermia. The strong expression of the inducible form of the hsp70 proteins and their nuclear location, like the more nucleolar presence of the hsc70 proteins seemed to confer heat resistance to the cells. Following minor morphological changes, the observation of the cytoskeleton network during stress recovery revealed a differential sensitivity of actin and tubuline to heat shock. The energy metabolism of both the microglial cell line and the monocyte cell line U937 were compared, under basal conditions, using phosphorus-31 NMR, to discern those phosphorylated metabolites which could be specific of either the monocytic or the macrophagic phenotype. A very high content of phosphocreatine was observed in the microglial clone whereas this compound was absent in monocytes. The lower CHME-5 phosphomonoester content as compared to monocytes also suggested that the microglial cell line displayed a more macrophagic metabolic pattern. After heat shock, the CHME-5 phosphorylated compounds showed large, transient, perturbations. Cells recovered their basal metabolic content 24 h after heat stress. This behaviour, in addition to the expression of inducible hsp70 proteins and the cytoskeleton rearrangement, are indicative of the particular adaptation of the microglial cell line to stress situations.
机译：小胶质细胞能够从静止状态转变为活化状态，在此状态下，它们获得了单核吞噬细胞原始形式以及这些细胞类型的高度分化形式所特有的功能。创伤后小胶质细胞的激活涉及功能的调整，包括基本细胞功能的复活和涉及细胞-细胞相互作用的功能的发展。在此，在热疗后观察到人小胶质细胞系CHME-5的行为。 hsp70蛋白的可诱导形式的强烈表达及其核位置，就像hsc70蛋白的核仁存在一样，似乎赋予了细胞耐热性。在轻微的形态变化后，在应力恢复过程中对细胞骨架网络的观察表明，肌动蛋白和微管蛋白对热休克的敏感性不同。在基础条件下，使用磷31 NMR比较了小胶质细胞系和单核细胞系U937的能量代谢，以识别那些可能对单核细胞或大噬细胞表型具有特异性的磷酸化代谢物。在小胶质细胞克隆中观察到磷酸肌酸的含量非常高，而在单核细胞中则没有这种化合物。与单核细胞相比，较低的CHME-5磷酸单酯含量也表明，小胶质细胞系显示出更大的巨噬细胞代谢模式。热冲击后，CHME-5磷酸化的化合物表现出较大的，短暂的扰动。热应激24小时后，细胞恢复了其基础代谢成分。除了可诱导的hsp70蛋白的表达和细胞骨架的重排之外，这种行为还表明了小胶质细胞系对应激情况的特殊适应性。

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