• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Metabotropic glutamate receptor/phospholipase C pathway is increased in rat brain at the end of pregnancy.
【24h】

Metabotropic glutamate receptor/phospholipase C pathway is increased in rat brain at the end of pregnancy.

机译：妊娠末期大鼠脑中代谢型谷氨酸受体/磷脂酶C途径增加。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Wistar pregnant rats were sacrificed at the end of pregnancy and the status of metabotropic glutamate receptors/phospholipase C (mGluR/PLC) pathway was studied in brain from pregnant and non-pregnant female rats. Pregnancy causes a significant increase in metabotropic glutamate receptors number, determined by radioligand binding assay, without significant changes on receptor affinity. Similar increase in mGluR(1) type was obtained by immunoblotting assay using specific anti-mGluR(1) antibody. However, no significant differences were observed in mGluR(5) type, suggesting that the increase detected by radioligand assays could be due to mGluR(1) up-regulation. On the other hand, a significant increase in the alpha subunit of G(q) protein was also detected in pregnant rats by immunoblotting assays. Real-time PCR experiments revealed a significant increase in gene expression of metabotropic glutamate receptors and G(q) proteins. Neither protein level nor gene expression of phospholipase C beta(1) isoform was altered in pregnant rats. However, an increase in basal and agonist-stimulated phospholipase C activity was observed in membranes from pregnant rats. These results suggest that gestational period causes the up-regulation of both metabotropic glutamate receptors and coupled G(q)-protein and, in turn, an increase in phospholipase C activity.
机译：Wistar妊娠大鼠在妊娠结束时被处死，并研究了妊娠和非妊娠雌性大鼠大脑中代谢型谷氨酸受体/磷脂酶C（mGluR / PLC）通路的状态。通过放射性配体结合测定法确定，怀孕会导致代谢型谷氨酸受体的数量显着增加，而受体亲和力却没有显着变化。使用特定的抗mGluR（1）抗体通过免疫印迹测定获得了类似的mGluR（1）类型增加。但是，没有观察到mGluR（5）类型的显着差异，这表明放射性配体测定法检测到的增加可能是由于mGluR（1）的上调所致。另一方面，通过免疫印迹测定法也可以在妊娠大鼠中检测到G（q）蛋白的α亚基显着增加。实时PCR实验表明，代谢型谷氨酸受体和G（q）蛋白的基因表达显着增加。磷脂酶C beta（1）亚型的蛋白质水平或基因表达均未改变。但是，在怀孕大鼠的膜中观察到了基础和激动剂刺激的磷脂酶C活性的增加。这些结果表明，妊娠期会引起代谢型谷氨酸受体和偶联的G（q）-蛋白的上调，进而导致磷脂酶C活性的增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号