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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >The distal carboxyl terminal of rat NR3B subunit regulates NR1-1a/NR3B and NR1-2a/NR3B surface trafficking.
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The distal carboxyl terminal of rat NR3B subunit regulates NR1-1a/NR3B and NR1-2a/NR3B surface trafficking.

机译：大鼠NR3B亚基的远端羧基末端调节NR1-1a / NR3B和NR1-2a / NR3B表面运输。

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摘要

N-Methyl-d-aspartate (NMDA) receptors are multi-subunit receptors formed from assembly of NR1 with NR2 and/or NR3 subunits. In this study, we investigated the role of a conserved RERLR motif present in a region within the distal carboxyl terminal of rat NR3B (between residues 952 and 984) in targeting NR1-1a/NR3B and NR1-2a/NR3B receptors to the cell surface. Surface biotinylation, confocal immunofluorescence microscopy and site-directed mutagenesis studies showed RERLR motif does not influence the surface expression of NR1-1a/NR3B NMDA receptor complex. Our bioinformatics analysis further showed this region can also exist as a coiled-coil domain. Truncation of this putative coiled-coil domain in NR3B affects surface expression of NR1-1a/NR3B and NR1-2a/NR3B receptors similarly suggesting that NR1 C1 cassette is not involved in the effect mediated by the distal carboxyl region of NR3B. This study represents the first attempt to evaluate a specific motif in regulating rat NR3B surface expression.
机译：N-甲基-d-天门冬氨酸（NMDA）受体是由NR1与NR2和/或NR3亚基组装而成的多亚基受体。在这项研究中，我们研究了大鼠NR3B远端羧基末端区域（残基952和984之间）中存在的保守RERLR基序在将NR1-1a / NR3B和NR1-2a / NR3B受体靶向细胞表面中的作用。表面生物素化，共聚焦免疫荧光显微镜和定点诱变研究表明RERLR基序不影响NR1-1a / NR3B NMDA受体复合物的表面表达。我们的生物信息学分析进一步表明，该区域也可以作为卷曲螺旋域存在。 NR3B中这个推定的卷曲螺旋结构域的截短会影响NR1-1a / NR3B和NR1-2a / NR3B受体的表面表达，类似地表明NR1 C1盒不参与NR3B远端羧基区域介导的作用。这项研究代表了首次尝试评估调节大鼠NR3B表面表达的特定基序。

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