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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Compartmentation of TCA cycle metabolism in cultured neocortical neurons revealed by 13C MR spectroscopy.
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Compartmentation of TCA cycle metabolism in cultured neocortical neurons revealed by 13C MR spectroscopy.

机译：通过13C MR光谱显示培养的新皮层神经元中TCA循环代谢的区室。

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摘要

Cultured neocortical neurons were incubated in medium containing [U-13C]glucose (0.5 mM) and in some cases unlabeled glutamine (0.5 mM). Subsequently the cells were "superfused" for investigation of the effect of depolarization by 55 mM K+. Cell extracts were analyzed by 13C magnetic resonance spectroscopy and gas chromatography/mass spectrometry to determine incorporation of 13C in glutamate, GABA, aspartate and fumarate. The importance of the tricarboxylic acid (TCA) cycle for conversion of the carbon skeleton of glutamine to GABA was evident from the effect of glutamine on the labeling pattern of GABA and glutamate. Moreover, analysis of the labeling patterns of glutamate in particular indicated a depolarization induced increased oxidative metabolism. This effect was only observed in glutamate and not in neurotransmitter GABA. Based on this a hypothesis of mitochondrial compartmentation may be proposed in which mitochondria associated with neurotransmitter synthesis are distinct from those aimed at energy production and influenced by depolarization. The hypothesis of mitochondrial compartmentation was further supported by the finding that the total percent labeling of fumarate and aspartate differed significantly from each other. This can only be explained by the existence of multiple TCA cycles with different turnover rates.
机译：将培养的新皮层神经元在含有[U-13C]葡萄糖（0.5 mM）和某些情况下未标记的谷氨酰胺（0.5 mM）的培养基中孵育。随后将细胞“融合”以研究55 mM K +对去极化的影响。通过13 C磁共振波谱和气相色谱/质谱法分析细胞提取物，以确定谷氨酸，GABA，天冬氨酸和富马酸酯中13 C的掺入。从谷氨酰胺对GABA和谷氨酸标记方式的影响中可以明显看出，三羧酸（TCA）循环对于将谷氨酰胺的碳骨架转化为GABA的重要性。此外，对谷氨酸标记模式的分析特别表明去极化诱导了氧化代谢的增加。仅在谷氨酸中观察到此作用，而在神经递质GABA中未观察到。基于此，可以提出线粒体区室化的假说，其中与神经递质合成相关的线粒体不同于旨在产生能量并受去极化影响的线粒体。发现富马酸酯和天冬氨酸的总百分比彼此显着不同的发现进一步支持了线粒体分区的假说。这只能通过存在具有不同周转率的多个TCA周期来解释。

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