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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Internalization and down-regulation of muscarinic acetylcholine receptors in cerebellar granule cells of tenascin-gene deficient mice.
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Internalization and down-regulation of muscarinic acetylcholine receptors in cerebellar granule cells of tenascin-gene deficient mice.

机译：肌腱蛋白基因缺陷小鼠小脑颗粒细胞中毒蕈碱型乙酰胆碱受体的内在化和下调。

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摘要

The expression of tenascin-C on oligodendrocytes parallels the migration of granule cells in the developing cerebellum, indicating a role for tenascin-C as a guide for granule neurons to find their proper locations. In this study, cultured cerebellar granule neurons from tenascin-C-knockout mice were used to examine the role of tenascin-C in agonist-induced muscarinic acetylcholine receptor down-regulation. Exposure of granule cells from wild-type or tenascin-C-negative mice to the muscarinic acetylcholine receptor agonist carbachol (1 mM) resulted in normal sequestration of cell-surface muscarinic acetylcholine receptors as assessed by [3H]N-methylscopolamine binding; however, down-regulation of total muscarinic acetylcholine receptors, measured with [3H]quinuclidinyl benzilate, was inhibited in granule cells from tenascin-C-negative mice. Remarkably, incubation of the tenascin-C-negative cells with the microtubule stabilizer taxol (10 microM) restored down-regulation of total muscarinic acetylcholine receptors to normal levels. We speculate that agonist-induced down-regulation of muscarinic acetylcholine receptors is functionally associated with tenascin-C-regulated microtubule structures in the developing cerebellum.
机译：腱生蛋白C在少突胶质细胞上的表达与颗粒细胞在发育中的小脑中的迁移平行，这表明腱生蛋白C作为指导颗粒神经元找到其适当位置的作用。在这项研究中，从腱糖蛋白C敲除小鼠培养的小脑颗粒神经元被用来检查腱糖蛋白C在激动剂诱导的毒蕈碱型乙酰胆碱受体下调中的作用。通过[3H] N-甲基东pol碱结合评估，野生型或肌腱蛋白-C阴性小鼠的颗粒细胞暴露于毒蕈碱乙酰胆碱受体激动剂卡巴胆碱（1 mM），可正常隔离细胞表面毒蕈碱乙酰胆碱受体。然而，用腱生蛋白-C-阴性小鼠的颗粒细胞抑制了总毒蕈碱乙酰胆碱受体的下调（用[3H]奎宁环戊基苯甲酸酯测量）。值得注意的是，肌腱蛋白C阴性细胞与微管稳定剂紫杉醇（10 microM）的孵育将总毒蕈碱乙酰胆碱受体的下调恢复到正常水平。我们推测激动剂引起的毒蕈碱乙酰胆碱受体的下调在功能上与正在发展的小脑中的肌腱蛋白-C调节的微管结构有关。

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