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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Acutely isolated astrocytes as models to probe astrocyte functions.
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Acutely isolated astrocytes as models to probe astrocyte functions.

机译：急性分离的星形胶质细胞作为探测星形胶质细胞功能的模型。

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Neuroscientists have become increasingly aware and accepting of the concept that astrocytes likely have many important functions in the CNS. One limitation in establishing these functions is the usual problem of what constitutes suitable experimental approaches. A major experimental step for functional studies of astrocytes has been the widespread use of primary astrocyte cultures, an approach that Leif Hertz pioneered. However, it is now becoming clear that, building on this work, an experimental paradigm shift is now needed. Namely, to increasingly study preparations corresponding to in situ conditions, such as slices. An alternative experimental system where the cells have some of the technical advantages of primary astrocyte cultures is freshly isolated astrocytes. Recent experiments from our laboratory have shown metabotropic glutamate receptor expression by such cells. Examples are given of how functional receptor studies and channel activity measured by patch clamp electrophysiology can be combined with single cell RT-PCR to define further the receptor or channel type are described to illustrate the uses of such preparations.
机译：神经科学家已经越来越意识到并接受星形胶质细胞可能在中枢神经系统中具有许多重要功能的概念。建立这些功能的一个限制是什么构成合适的实验方法的常见问题。星形胶质细胞功能性研究的主要实验步骤是原代星形胶质细胞培养物的广泛使用，这是莱夫·赫兹（Leif Hertz）率先提出的方法。但是，现在越来越清楚的是，在这项工作的基础上，现在需要实验性的范式转换。即，越来越多地研究与原位条件相对应的制剂，例如切片。细胞具有原代星形胶质细胞培养的某些技术优势的另一种实验系统是新鲜分离的星形胶质细胞。我们实验室最近的实验表明这类细胞可代谢谷氨酸受体。给出了如何将功能性受体研究和通过膜片钳电生理学测量的通道活性与单细胞RT-PCR结合以进一步定义受体或通道类型的例子，以说明此类制剂的用途。

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