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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Distribution of plasma membrane Ca2+ pump (PMCA) isoforms in the gerbil brain: effect of ischemia-reperfusion injury.
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Distribution of plasma membrane Ca2+ pump (PMCA) isoforms in the gerbil brain: effect of ischemia-reperfusion injury.

机译：沙土鼠脑中质膜Ca2 +泵（PMCA）异构体的分布：缺血-再灌注损伤的作用。

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摘要

Non-species isoform-specific antibodies against three isoforms of the plasma membrane Ca2+ pump (PMCA) were used for immuno-localization of PMCA by Western blot analysis in membrane preparations isolated from different regions of gerbil brain. All three gene products were detected in the membranes from hippocampus, cerebral cortex and cerebellum. However, they showed a distinct distribution pattern. Two proteins were revealed in the case of PMCA1 with molecular masses 129 and 135 kDa. The antibody against PMCA2 recognized three proteins of about 130-137 kDa. Only one protein was detected with the anti-PMCA3 antibody. Levels of immuno-signal for the PMCA isoforms varied significantly among the different brain regions. The PMCA1 is the most abundant in the cerebro-cortical and hippocampal membrane preparations. The PMCA2 was detected in a lesser amount comparing to PMCA1 and was highest in the membrane preparations from cerebellum and in a slightly lesser amount from cerebral cortex. Anti-PMCA3 antibody stained weakly and was localized in the cerebellar and hippocampal membrane preparations. Transient forebrain ischemia (10 min) and reperfusion (for a prolonged period up to 10 d) leads to a significant decrease of PMCA immuno-signal. This decrease could be ascribed to the loss of PMCA1 signal, especially in hippocampal membrane preparations.
机译：针对来自沙土鼠不同区域的膜制剂，通过蛋白质印迹分析，针对浆膜Ca2 +泵（PMCA）三种同工型的非种同工型特异性抗体用于PMCA的免疫定位。在海马，大脑皮层和小脑的膜中检测到所有三个基因产物。但是，它们显示出独特的分布模式。在PMCA1中发现了两种蛋白质，分子量分别为129和135 kDa。抗PMCA2的抗体可识别约130-137 kDa的三种蛋白质。抗PMCA3抗体仅检测到一种蛋白质。在不同的大脑区域之间，PMCA亚型的免疫信号水平差异很大。 PMCA1在脑皮质和海马膜制剂中含量最高。与PMCA1相比，检测到的PMCA2含量要少，在小脑的膜制剂中检测到的含量最高，而从大脑皮层中检测到的含量要少一些。抗PMCA3抗体染色较弱，位于小脑和海马膜制剂中。短暂性前脑缺血（10分钟）和再灌注（长达10 d的长时间）导致PMCA免疫信号显着降低。这种下降可能归因于PMCA1信号的丢失，尤其是在海马膜制剂中。

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