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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Modest cholinergic deafferentation fails to alter hippocampal G-proteins.
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Modest cholinergic deafferentation fails to alter hippocampal G-proteins.

机译：适度的胆碱能脱除咖啡因不能改变海马G蛋白。

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摘要

The integrity of hippocampal G-protein mediated signalling following ibotenate induced lesion of the medial septum was examined. The lesion was confined histologically to the septum and induced a 23% reduction in hippocampal choline acetyltransferase (ChAT) activity and G-proteins levels and related enzyme activities were measured in the hippocampus following a 21 day survival period. The relative levels of five G-protein subunits (Gbeta, G(alpha)o, G(alpha)i1, G(alpha)i2, and G(alpha)s-L), basal GTPase, the degree of carbachol- or baclofen-stimulated GTPase activities, and the basal and fluoroaluminate-stimulated adenylate cyclase activities were apparently unaffected. To determine if our assay methodology was sensitive to changes in pre-synaptic signalling, we compared G-protein density in synaptosomes with total hippocampal homogenates. The concentration of G(alpha)q/11, G(alpha)i1, and G(alpha)i2. were significantly lower in synaptosomes, while G(alpha)o, was only marginally reduced. Thus, modest lesions of the medial-septal nucleus fail to alter G-protein signalling. However, our findings that G-protein density is lower in synaptosomal membranes than in total homogenates, indicates that the analysis of signalling events in synaptosomes following deafferentation could clarify adaptive changes which may occur at the presynaptic level.
机译：检查了ibotenate诱导的内侧中隔病变后海马G蛋白介导的信号传导的完整性。病变在组织学上仅限于隔膜，并导致海马胆碱乙酰转移酶（ChAT）活性降低了23％，存活21天后在海马中测量了G蛋白水平和相关的酶活性。五个G蛋白亚基（Gbeta，Gαo，Gαi1，Gαi2和GαssL）的相对水平，基础GTP酶，卡巴酚或巴氯酚刺激的程度GTPase活性以及基础和氟铝酸盐刺激的腺苷酸环化酶活性显然未受影响。为了确定我们的检测方法是否对突触前信号的变化敏感，我们比较了突触体和总海马匀浆中的G蛋白密度。 Gαq/ 11，Gαi1和Gαi2的浓度。突触小体中的α明显降低，而Gαo仅略微降低。因此，内侧中隔核的适度损害不能改变G蛋白信号传导。但是，我们的发现表明突触体膜中的G蛋白密度低于总匀浆中的G蛋白密度，这表明对脱除咖啡因后突触小体中信号传递事件的分析可以阐明可能发生在突触前水平的适应性变化。

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