Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.

Evans SV; Sigurskjold BW; Jennings HJ; Brisson JR; To R; Tse WC; Altman E; Frosch M; Weisgerber C; Kratzin HD; al.

首页> 外文期刊>Biochemistry >Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.

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Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.

机译：多糖表位的扩展螺旋性质的证据。 2.8对α-（2-> 8）-多唾液酸具有特异性的抗原结合片段的配体结合的拆分结构和热力学。

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The antigen binding fragment from an IgG2a kappa murine monoclonal antibody with specificity for alpha-(2-->8)-linked sialic acid polymers has been prepared and crystallized in the absence of hapten. Crystals were grown by vapor diffusion equilibrium with 16-18% polyethylene glycol 4000 solutions. The structure was solved by molecular replacement methods and refined to a conventional R factor of 0.164 for data to 2.8 A. The binding site is observed to display a shape and distribution of charges that is complementary to that of the predicted conformation of the oligosaccharide epitope. A thermodynamic description of ligand binding has been compiled for oligosaccharides ranging in length from 9 to 41 residues, and the data for the largest ligand has been used in a novel way to estimate the size of the antigen binding site. A model of antigen binding is presented that satisfies this thermodynamic data, as well as a previously reported requirement of conformational specificity of the oligosaccharide. X-raycrystallographic and thermodynamic evidence are consistent with a binding site that accommodates at least eight sialic acid residues.

机译：已经制备了对α-（2-→8）连接的唾液酸聚合物具有特异性的IgG2aκ鼠单克隆抗体的抗原结合片段，并在没有半抗原的情况下使其结晶。通过用16-18％聚乙二醇4000溶液进行蒸气扩散平衡来生长晶体。通过分子置换方法解决了结构问题，并将其精炼为常规R因子0.164，从而获得了2.8 A的数据。观察到结合位点显示出与寡糖表位的预期构型互补的电荷形状和分布。已经编辑了长度为9至41个残基的寡糖的配体结合的热力学描述，并且已经以新颖的方式使用了最大配体的数据来估计抗原结合位点的大小。提出了满足该热力学数据以及先前报道的寡糖构象特异性要求的抗原结合模型。 X射线晶体学和热力学证据与容纳至少八个唾液酸残基的结合位点一致。

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《Biochemistry》 |1995年第20期|共8页
作者
Evans SV; Sigurskjold BW; Jennings HJ; Brisson JR; To R; Tse WC; Altman E; Frosch M; Weisgerber C; Kratzin HD; al.;
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正文语种 eng
中图分类生物化学;
关键词
Antibodies; Monoclonal; Epitopes; Peptide Fragments; Protein Structure; Secondary; 抗体; 单克隆; 表位; 肽碎片; 蛋白质结构; 二级; 唾液酸类;

机译：Antibodies;Monoclonal;Epitopes;Peptide Fragments;Protein Structure;Secondary;抗体;单克隆;表位;肽碎片;蛋白质结构;二级;唾液酸类;

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1. Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid. [J] . Evans SV, Sigurskjold BW, Jennings HJ, Biochemistry . 1995,第20期

机译：多糖表位的扩展螺旋性质的证据。 2.8对α-（2-> 8）-多唾液酸具有特异性的抗原结合片段的配体结合的拆分结构和热力学。
2. Allosteric properties of inosine monophosphate dehydrogenase revealed through the thermodynamics of binding of inosine 5'-monophosphate and mycophenolic acid. Temperature dependent heat capacity of binding as a signature of ligand-coupled conformatio [J] . Bruzzese FJ, Connelly PR Biochemistry . 1997,第34期

机译：Inosine单磷酸脱氢酶的变构特性通过Inosine 5'-单磷酸肌酐结合的热力学揭示。温度依赖性热量的结合作为配体耦合的符合性的签名
3. Monoclonal antibodies specific for O-antigenic polysaccharides of Shigella flexneri: clones binding to II, II:3,4, and 7,8 epitopes. [J] . N I Carlin, A A Lindberg Journal of Clinical Microbiology . 1983,第5期

机译：对弗氏志贺氏菌O抗原多糖具有特异性的单克隆抗体：与II，II：3、4和7,8表位结合的克隆。
4. Monoclonal antibodies specific for O-antigenic polysaccharides of Shigella flexneri: clones binding to II II:34 and 78 epitopes. [O] . N I Carlin, A A Lindberg 1983

机译：对弗氏志贺氏菌O抗原多糖具有特异性的单克隆抗体：与IIII：3、4和78表位结合的克隆。
5. Crystal structure of the antigen-binding fragment of the murine anti-arsonate monoclonal antibody 36-71 at 2.9-A resolution. [O] . Rose, D R, Strong, R K, Margolies, M N, 1990

机译：鼠抗砷酸盐单克隆抗体36-71的抗原结合片段的晶体结构，分辨率为2.9-A。

Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.

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