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The first stage of cardinal direction selectivity is localized to the dendrites of retinal ganglion cells

机译:基本方向选择性的第一阶段定位于视网膜神经节细胞的树突

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摘要

Inferring the direction of image motion is a fundamental component of visual computation and essential for visually guided behavior. In the retina, the direction of image motion is computed in four cardinal directions, but it is not known at which circuit location along the flow of visual information the cardinal direction selectivity first appears. We recorded the concerted activity of the neuronal circuit elements of single direction-selective (DS) retinal ganglion cells at subcellular resolution by combining GCaMP3-functionalized transsynaptic viral tracing and two-photon imaging. While the visually evoked activity of the dendritic segments of the DS cells were direction selective, direction-selective activity was absent in the axon terminals of bipolar cells. Furthermore, the glutamate input to DS cells, recorded using a genetically encoded glutamate sensor, also lacked direction selectivity. Therefore, the first stage in which extraction of a cardinal motion direction occurs is the dendrites of DS cells.
机译:推断图像运动的方向是视觉计算的基本组成部分,对于视觉引导的行为至关重要。在视网膜中,图像运动的方向是沿四个基本方向计算的,但尚不知道基本方向选择性首先出现在沿视觉信息流的哪个电路位置。我们通过结合GCaMP3功能化的突触病毒示踪和双光子成像,记录了亚方向分辨率的单向选择性(DS)视网膜神经节细胞的神经元电路元件的协调活动。尽管DS细胞的树突状片段的视觉诱发的活性是方向选择性的,但双极细胞的轴突末端不存在方向选择性的活性。此外,使用遗传编码的谷氨酸传感器记录到DS细胞的谷氨酸输入也缺乏方向选择性。因此,发生基本运动方向提取的第一阶段是DS细胞的树突。

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