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首页> 外文期刊>Neuron >Multimerization of the ligand binding domains of cyclic nucleotide-gated channels.
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Multimerization of the ligand binding domains of cyclic nucleotide-gated channels.

机译：环状核苷酸门控通道的配体结合域的多聚化。

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摘要

Cyclic nucleotide-gated (CNG) channels comprise four subunits and are activated by the direct binding of cyclic nucleotide to an intracellular domain on each subunit. This ligand binding domain is thought to contain a beta roll followed by two alpha helices, designated the B and C helices. To examine the quaternary structure of CNG channels and how it changes during ion channel gating, we introduced single cysteines along the C helix of each subunit in an otherwise cysteineless channel. We found that cysteines on the C helices could form intersubunit disulfide bonds, even between diagonal subunits. Disulfide bond formation occurred primarily in closed channels and inhibited channel opening. These data suggest that the C helices from all four channel subunits are in close proximity in the closed state and move apart during channel opening.
机译：环状核苷酸门控（CNG）通道包含四个亚基，并通过环状核苷酸与每个亚基上的细胞内结构域的直接结合而被激活。该配体结合结构域被认为包含β卷，其后是两个α螺旋，称为B和C螺旋。为了检查CNG通道的四级结构及其在离子通道门控期间的变化，我们沿无半胱氨酸通道中每个亚基的C螺旋引入了单个半胱氨酸。我们发现，C螺旋上的半胱氨酸即使在对角亚基之间也可以形成亚基间二硫键。二硫键的形成主要发生在封闭通道中，并抑制了通道的开放。这些数据表明来自所有四个通道亚基的C螺旋在关闭状态下非常接近，并且在通道打开过程中分开。

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