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首页> 外文期刊>Neuron >Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-term facilitation in Aplysia.
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Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-term facilitation in Aplysia.

机译：需要持续的CPEB依赖的局部蛋白合成来稳定突触生长，以持久维持海藻的长期生长。

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摘要

The time course of the requirement for local protein synthesis in the stabilization of learning-related synaptic growth and the persistence of long-term memory was examined using Aplysia bifurcated sensory neuron-motor neuron cultures. We find that, following repeated pulses of serotonin (5-HT), the local perfusion of emetine, an inhibitor of protein synthesis, or a TAT-AS oligonucleotide directed against ApCPEB blocks long-term facilitation (LTF) at either 24 or 48 hr and leads to a selective retraction of newly formed sensory neuron varicosities induced by 5-HT. By contrast, later inhibition of local protein synthesis, at 72 hr after 5-HT, has no effect on either synaptic growth or LTF. These results define a specific stabilization phase for the storage of long-term memory during which newly formed varicosities are labile and require sustained CPEB-dependent local protein synthesis to acquire the more stable properties of mature varicosities required for the persistence of LTF.
机译：使用Aplysia分叉的感觉神经元-运动神经元培养物检查了学习相关突触生长的稳定和长期记忆持久性中局部蛋白质合成需求的时间过程。我们发现，继5-羟色胺（5-HT）的重复脉冲后，左美汀，蛋白质合成抑制剂或针对ApCPEB的TAT-AS寡核苷酸的局部灌注会在24或48小时处阻断长期促进（LTF）并导致由5-HT诱导的新形成的感觉神经元静脉曲张选择性收缩。相比之下，在5-HT后72小时抑制局部蛋白质合成对突触生长或LTF均无影响。这些结果为长期记忆的存储定义了一个特定的稳定阶段，在此阶段中，新形成的静脉曲张不稳定，需要持续的CPEB依赖性局部蛋白质合成，以获得LTF持久性所需的成熟静脉曲张的更稳定的特性。

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