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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Transplanted human embryonic neural stem cells survive, migrate, differentiate and increase endogenous nestin expression in adult rat cortical peri-infarction zone.
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Transplanted human embryonic neural stem cells survive, migrate, differentiate and increase endogenous nestin expression in adult rat cortical peri-infarction zone.

机译：移植的人类胚胎神经干细胞在成年大鼠皮质梗死区中存活，迁移，分化并增加内源巢蛋白表达。

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摘要

Transplantation of stem cells is a potential therapeutic strategy for stroke damage. The survival, migration, and differentiation of transplanted human embryonic neural stem cells in the acute post-ischemic environment were characterized and endogenous nestin expression after transplantation was investigated. Human embryonic neural stem cells obtained from the temporal lobe cortex were cultured and labeled with fluorescent 1,1'-dioctadecy-6,6'-di (4-sulfopheyl)-3,3,3',3'-tetramethylindocarbocyanin (DiI) in vitro. Labeled cells were transplanted into cortical peri-infarction zones of adult rats 24 h after permanent middle cerebral artery occlusion. Survival, migration, and differentiation of grafted cells were quantified in immunofluorescence-stained sections from rats sacrificed at 7, 14, and 28 days after transplantation. Endogenous nestin-positive cells in the cortical peri-infarction zone were counted at serial time points. The cells transplanted into the cortical peri-infarction zone displayed the morphology of living cells and became widely located around the ischemic area. Moreover, some of the transplanted cells expressed nestin, GFAP, or NeuN in the peri-infarction zone. Furthermore, compared with the control group, endogenous nestin-positive cells in the peri-infarction zone had increased significantly 7 days after cell transplantation. These results confirm the survival, migration, and differentiation of transplanted cells in the acute post-ischemic environment and enhanced endogenous nestin expression within a brief time window. These findings indicate that transplantation of neural stem cells into the peri-infarction zone may be performed as early as 24 h after ischemia.
机译：干细胞移植是中风损伤的潜在治疗策略。表征了急性缺血后环境中移植的人类胚胎神经干细胞的存活，迁移和分化，并研究了移植后内源性巢蛋白的表达。培养从颞叶皮层获得的人类胚胎神经干细胞，并用荧光1,1'-二十八烷基-6,6'-二（4-巯基）-3,3,3'，3'-四甲基吲哚碳花青素（DiI）标记体外。永久性大脑中动脉闭塞24小时后，将标记的细胞移植到成年大鼠的皮质梗死区。在移植后第7、14和28天处死的大鼠的免疫荧光染色切片中，对移植细胞的存活，迁移和分化进行了定量。在连续的时间点计数皮层梗死区的内源性巢蛋白阳性细胞。移植到皮层梗死区的细胞表现出活细胞的形态，并在缺血区域周围广泛分布。此外，一些移植的细胞在梗塞周围区域表达巢蛋白，GFAP或NeuN。此外，与对照组相比，梗死周围区域的内源性巢蛋白阳性细胞在细胞移植后7天明显增加。这些结果证实了移植后细胞在急性缺血后环境中的存活，迁移和分化，并在短时间范围内增强了内源性巢蛋白的表达。这些发现表明，神经干细胞可以在缺血后24小时内移植到梗塞周围区域。

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