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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >The positive correlation between DJ-1 and β-catenin expression shows prognostic value for patients with glioma.
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The positive correlation between DJ-1 and β-catenin expression shows prognostic value for patients with glioma.

机译：DJ-1和β-catenin表达之间的正相关性显示了脑胶质瘤患者的预后价值。

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The relationship between DJ-1 and β-catenin, and its impact on the prognosis for glioma patients has not been fully understood. This study determined the effect of DJ-1 on β-catenin and the prognostic significance of this interaction in glioma patients. We collected tumor specimens from 88 glioma patients and determined the expression of DJ-1, β-catenin and PTEN by using immunohistochemical staining. The involvement of DJ-1 and β-catenin in glioma cell lines was evaluated by immunohistochemistry and Western blotting. High DJ-1 expression (37.5%) and high β-catenin expression (34.1%) in glioma specimens were significantly associated with high grade and poor prognosis in glioma patients. However, only high levels of DJ-1 (P = 0.014) was a strong independent prognostic factor, correlated with a reduced overall survival time. In vitro?DJ-1 expression was positively correlated with the expression levels of β-catenin and p-Akt, and negatively correlated with PTEN expression in U87, U251 MG, SWO-38 and SHG44 human glioma cell lines. After the knockdown of DJ-1, Akt, p-Akt or β-catenin expression levels were not affected in the PTEN-null cell lines (U87 and U251 MG). However, in the SWO-38 cell line, which has wild-type PTEN protein, the level of PTEN increased while Akt/p-Akt and β-catenin levels were reduced. Furthermore, β-catenin staining weakened in SWO-38 cells after DJ-1 levels decreased according to immunocytochemical analysis. In conclusion, DJ-1 and β-catenin may contribute to the development and recurrence of glioma and are valuable prognostic factors for glioma patients. DJ-1 may regulate β-catenin expression via PTEN and p-Akt.
机译：DJ-1和β-catenin之间的关系及其对神经胶质瘤患者预后的影响尚未完全了解。这项研究确定了DJ-1对β-catenin的影响以及这种相互作用在神经胶质瘤患者中的预后意义。我们收集了88例神经胶质瘤患者的肿瘤标本，并通过免疫组织化学染色确定了DJ-1，β-catenin和PTEN的表达。通过免疫组织化学和蛋白质印迹评估DJ-1和β-连环蛋白在神经胶质瘤细胞系中的参与。脑胶质瘤标本中高DJ-1表达（37.5％）和高β-catenin表达（34.1％）与脑胶质瘤患者的高分级和不良预后密切相关。然而，只有高水平的DJ-1（P = 0.014）是很强的独立预后因素，与减少的总生存时间有关。 UJ，U251 MG，SWO-38和SHG44人神经胶质瘤细胞系中DJ-1的体外表达与β-catenin和p-Akt的表达呈正相关，与PTEN的表达呈负相关。敲低DJ-1后，在PTEN无效的细胞系（U87和U251 MG）中，Akt，p-Akt或β-catenin的表达水平不受影响。但是，在具有野生型PTEN蛋白的SWO-38细胞系中，PTEN的水平升高，而Akt / p-Akt和β-catenin的水平降低。此外，根据免疫细胞化学分析，DJ-1水平降低后，SWO-38细胞中的β-catenin染色减弱。总之，DJ-1和β-catenin可能促进神经胶质瘤的发展和复发，是神经胶质瘤患者的重要预后因素。 DJ-1可能通过PTEN和p-Akt调节β-catenin的表达。

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