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首页> 外文期刊>Neuron >Conformational rearrangements associated with the gating of the G protein-coupled potassium channel revealed by FRET microscopy.
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Conformational rearrangements associated with the gating of the G protein-coupled potassium channel revealed by FRET microscopy.

机译：FRET显微镜显示与G蛋白偶联钾通道的门控相关的构象重排。

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摘要

G protein-coupled potassium channels (GIRK/Kir3.x) are key determinants that translate inhibitory chemical neurotransmission into changes in cellular excitability. To understand the mechanism of channel activation by G proteins, it is necessary to define the structural rearrangements in the channel that result from interaction with Gbetagamma subunits. In this study we used a combination of fluorescence spectroscopy and through-the-objective total internal reflection microscopy to monitor the conformational rearrangements associated with the activation of GIRK channels in single intact cells. We detect activation-induced changes in FRET consistent with a rotation and expansion of the termini along the central axis of the channel. We propose that this rotation and expansion of the termini drives the channel to open by bending and possibly rotating the second transmembrane segment.
机译：G蛋白偶联的钾离子通道（GIRK / Kir3.x）是将抑制性化学神经传递转化为细胞兴奋性变化的关键决定因素。要了解G蛋白激活通道的机制，有必要定义通道中由于与Gbetagamma亚基相互作用而引起的结构重排。在这项研究中，我们结合了荧光光谱法和客观全内反射显微镜技术，以监测与单个完整细胞中GIRK通道激活相关的构象重排。我们检测到激活诱导的FRET变化与沿通道中心轴的末端旋转和扩展一致。我们建议末端的旋转和扩展通过弯曲并可能旋转第二个跨膜段来驱动通道打开。

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