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首页> 外文期刊>Neuron >Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse.
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Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse.

机译：Ca2 +通道与Ca2 +传感器之间的纳米域耦合促进了皮质GABA能突触的快速有效释放。

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摘要

It is generally thought that transmitter release at mammalian central synapses is triggered by Ca2+ microdomains, implying loose coupling between presynaptic Ca2+ channels and Ca2+ sensors of exocytosis. Here we show that Ca2+ channel subunit immunoreactivity is highly concentrated in the active zone of GABAergic presynaptic terminals of putative parvalbumin-containing basket cells in the hippocampus. Paired recording combined with presynaptic patch pipette perfusion revealed that GABA release at basket cell-granule cell synapses is sensitive to millimolar concentrations of the fast Ca2+ chelator BAPTA but insensitive to the slow Ca2+ chelator EGTA. These results show that Ca2+ source and Ca2+ sensor are tightly coupled at this synapse, with distances in the range of 10-20 nm. Models of Ca2+ inflow-exocytosis coupling further reveal that the tightness of coupling increases efficacy, speed, and temporal precision of transmitter release. Thus, tight coupling contributes to fast feedforward and feedback inhibition in the hippocampal network.
机译：通常认为，哺乳动物中枢突触的递质释放是由Ca2 +微域触发的，这意味着突触前Ca2 +通道与胞吐作用的Ca2 +传感器之间存在松散耦合。在这里，我们显示，Ca2 +通道亚基的免疫反应性高度集中在海马中假定的含小白蛋白的篮子细胞的GABA能突触前末端的活性区中。配对记录与突触前贴片吸液灌注相结合，揭示了篮状细胞-颗粒细胞突触中的GABA释放对快速Ca2 +螯合剂BAPTA的毫摩尔浓度敏感，但对慢速Ca2 +螯合剂EGTA不敏感。这些结果表明，Ca2 +源和Ca2 +传感器在此突触处紧密耦合，距离在10-20 nm之间。 Ca 2+流入-胞吐作用偶联的模型进一步揭示，偶联的紧密性增加了递质释放的功效，速度和时间精度。因此，紧密的耦合有助于海马网络中的快速前馈和反馈抑制。

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