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首页> 外文期刊>Neuron >Pathway-specific feedforward circuits between thalamus and neocortex revealed by selective optical stimulation of axons.
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Pathway-specific feedforward circuits between thalamus and neocortex revealed by selective optical stimulation of axons.

机译：丘脑和新皮层之间的特定于通路的前馈回路通过轴突的选择性光学刺激得以揭示。

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摘要

Thalamocortical and corticothalamic pathways mediate bidirectional communication between the thalamus and neocortex. These pathways are entwined, making their study challenging. Here we used lentiviruses to express channelrhodopsin-2 (ChR2), a light-sensitive cation channel, in either thalamocortical or corticothalamic projection cells. Infection occurred only locally, but efferent axons and their terminals expressed ChR2 strongly, allowing selective optical activation of each pathway. Laser stimulation of ChR2-expressing thalamocortical axons/terminals evoked robust synaptic responses in cortical excitatory cells and fast-spiking (FS) inhibitory interneurons, but only weak responses in somatostatin-containing interneurons. Strong FS cell activation led to feedforward inhibition in all cortical neuron types, including FS cells. Corticothalamic stimulation excited thalamic relay cells and inhibitory neurons of the thalamic reticular nucleus (TRN). TRN activation triggered inhibition in relay cells but not in TRN neurons. Thus, a major difference between thalamocortical and corticothalamic processing was the extent to which feedforward inhibitory neurons were themselves engaged by feedforward inhibition.
机译：丘脑皮质和皮质丘脑途径介导丘脑和新皮层之间的双向通讯。这些途径纠缠在一起，使他们的学习充满挑战。在这里，我们使用慢病毒在丘脑皮层或皮层丘脑投射细胞中表达光敏阳离子通道Channelrhodopsin-2（ChR2）。感染仅发生在局部，但是传出的轴突及其末端强烈表达ChR2，从而允许每个途径的选择性光学激活。激光刺激表达ChR2的丘脑皮层轴突/末端在皮层兴奋性细胞和快速加标（FS）抑制性中间神经元中引起强烈的突触反应，但在含有生长抑素的中间神经元中仅弱反应。强烈的FS细胞激活导致所有皮质神经元类型（包括FS细胞）的前馈抑制。皮质丘脑刺激刺激丘脑中继细胞和丘脑网状核（TRN）的抑制神经元。 TRN激活触发了中继细胞中的抑制，但未触发TRN神经元中的抑制。因此，丘脑皮质和皮质丘脑加工之间的主要区别是前馈抑制神经元自身通过前馈抑制作用的程度。

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