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首页> 外文期刊>Neuron >Imaging voltage in neurons.
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Imaging voltage in neurons.

机译:神经元中的成像电压。

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摘要

In the last decades, imaging membrane potential has become a fruitful approach to study neural circuits, especially in invertebrate preparations with large, resilient neurons. At the same time, particularly in mammalian preparations, voltage imaging methods suffer from poor signal to noise and secondary side effects, and they fall short of providing single-cell resolution when imaging of the activity of neuronal populations. As an introduction to these techniques, we briefly review different voltage imaging methods (including organic fluorophores, SHG chromophores, genetic indicators, hybrid, nanoparticles, and intrinsic approaches) and illustrate some of their applications to neuronal biophysics and mammalian circuit analysis. We discuss their mechanisms of voltage sensitivity, from reorientation, electrochromic, or electro-optical phenomena to interaction among chromophores or membrane scattering, and highlight their advantages and shortcomings, commenting on the outlook for development of novel voltage imaging methods.
机译:在过去的几十年中,对膜电位进行成像已成为研究神经回路的有效方法,尤其是在具有大型弹性神经元的无脊椎动物制剂中。同时,特别是在哺乳动物制剂中,电压成像方法具有差的信噪比和次级副作用,并且当对神经元群体的活性成像时,它们不能提供单细胞分辨率。作为对这些技术的介绍,我们简要回顾了不同的电压成像方法(包括有机荧光团,SHG生色团,遗传指示剂,杂交体,纳米颗粒和固有方法),并说明了它们在神经元生物物理学和哺乳动物电路分析中的一些应用。我们讨论了它们的电压敏感性机制,从重新定向,电致变色或电光现象到生色团之间或膜散射之间的相互作用,并突出了它们的优点和缺点,并评论了新型电压成像方法的发展前景。

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