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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Fibrin matrix provides a suitable scaffold for bone marrow stromal cells transplanted into injured spinal cord: a novel material for CNS tissue engineering.
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Fibrin matrix provides a suitable scaffold for bone marrow stromal cells transplanted into injured spinal cord: a novel material for CNS tissue engineering.

机译：纤维蛋白基质为移植到受损脊髓中的骨髓基质细胞提供了合适的支架：一种用于中枢神经系统组织工程的新型材料。

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Recent basic experiments have strongly suggested that cell transplantation therapy may promote functional recovery in patients with spinal cord injury (SCI). However, a safe and efficient transplantation technique still remains undetermined. This study, therefore, was aimed to clarify whether fibrin matrix could be a useful scaffold in bone marrow stromal cell (BMSC) transplantation for the injured spinal cord. To clarify the issue, three-dimensional structure of fibrin matrix was assessed and the green fluorescent protein (GFP)-expressing BMSC were cultured in fibrin matrix. The rats were subjected to spinal cord hemisection at T8 level, and the vehicle, BMSC or BMSC-fibrin matrix construct was implanted into the cavity. Neurologic function was serially evaluated. Using immunohistochemistry, we evaluated the survival, migration and differentiation of the transplanted cells at 4 weeks after transplantation. In the initial in vitro study, the BMSC could survive in fibrin matrix for 2 weeks. The animals treated with the BMSC-fibrin matrix construct showed significantly more pronounced recovery of neurologic function than vehicle- or BMSC-treated animals. Fibrin scaffold markedly improved the survival and migration of the transplanted cells. There was no significant difference in the percentage of cells doubly positive for GFP and microtubule-associated protein 2 between the animals treated with BMSC-fibrin matrix construct and those treated with BMSC, but a certain subpopulation of GFP-positive cells morphologically simulated the neurons in the animals treated with BMSC-fibrin matrix construct. These findings strongly suggest that fibrin matrix may be one of the promising candidates for a potential, minimally invasive scaffold for injured spinal cord, and that such strategy of tissue engineering could be a hopeful option in regeneration therapy for patients with SCI.
机译：最近的基础实验强烈建议，细胞移植疗法可促进脊髓损伤（SCI）患者的功能恢复。但是，安全和有效的移植技术仍然不确定。因此，本研究旨在阐明纤维蛋白基质是否可能在骨髓基质细胞（BMSC）移植中用于损伤脊髓的有用支架。为了澄清这个问题，评估了纤维蛋白基质的三维结构，并在纤维蛋白基质中培养了表达绿色荧光蛋白（GFP）的BMSC。将大鼠在T8水平进行脊髓半切，并将媒介物BMSC或BMSC-纤维蛋白基质构建体植入腔中。连续评估神经功能。使用免疫组织化学，我们评估了移植后4周的移植细胞的存活，迁移和分化。在最初的体外研究中，BMSC可以在纤维蛋白基质中存活2周。与媒介物或BMSC处理的动物相比，用BMSC纤维蛋白基质构建物处理的动物表现出明显更强的神经功能恢复。纤维蛋白支架显着改善了移植细胞的存活和迁移。用BMSC-纤维蛋白基质构建物处理的动物与用BMSC处理的动物在GFP和微管相关蛋白2双重阳性细胞百分比上没有显着差异，但是GFP阳性细胞的某些亚群在形态学上模拟了神经元。用BMSC-纤维蛋白基质构建体处理的动物。这些发现强烈表明，纤维蛋白基质可能是潜在的，潜在的损伤性脊髓微创支架的有前途的候选者之一，并且这种组织工程学策略可能是SCI患者再生治疗中的一种有希望的选择。

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