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首页> 外文期刊>Neuron >Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.
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Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.

机译：活动诱导的突触成熟由突触前cdc42信号介导。

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摘要

Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in the number of depolarization-induced FM4-64 puncta in the presynaptic axon. This rapid presynaptic maturation can be abolished by treatments that interfered with presynaptic BDNF and Cdc42 signaling or actin polymerization. Activation of Cdc42 by applying BDNF or bradykinin mimicked the effect of electrical activity in promoting synaptic maturation. Furthermore, activity-induced increase in presynaptic actin polymerization, as revealed by increased concentration of actin-YFP at axon boutons, was abolished by inhibiting BDNF and Cdc42 signaling. Thus, rapid presynaptic maturation induced by neuronal activity is mediated by presynaptic activation of the Cdc42 signaling pathway.
机译：突触前递质分泌机制的成熟是突触发生的关键步骤。在这里我们报告说，突触前动作电位的简短训练通过增强突触前谷氨酸的释放，将培养的海马神经元之间的早期非功能性接触迅速转变为功能性突触。突触前轴突中去极化诱导的FM4-64突点数量明显增加证实了释放的增强。这种快速的突触前成熟可以通过干扰突触前B​​DNF和Cdc42信号转导或肌动蛋白聚合的治疗来消除。通过应用BDNF或缓激肽激活Cdc42可以模仿电活动促进突触成熟的作用。此外，通过抑制BDNF和Cdc42信号信号，消除了活性诱导的突触前肌动蛋白聚合反应的增加，如轴突纽扣处肌动蛋白YFP浓度的增加所揭示的。因此，由神经元活动诱导的突触前快速成熟是由Cdc42信号通路的突触前激活介导的。

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