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首页> 外文期刊>Neuron >Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses
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Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses

机译：突触小泡对接位点的分子分析揭示了新型蛋白质，但谷氨酸能和GABA能突触之间几乎没有差异

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Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically isolate fractions highly enriched in docked synaptic vesicles. The fraction is largely free of postsynaptic proteins and most other organelles while containing most known synaptic vesicle and active zone proteins. Numerous presynaptic transmembrane proteins were also identified, together with over 30 uncharacterized proteins, many of which are evolutionarily conserved. Quantitative proteomic comparison of glutamate- and GABA-specific docking complexes revealed that, except of neurotransmitter-specific enzymes and transporters, only few proteins were selectively enriched in either fraction. We conclude that the core machinery involved in vesicle docking and exocytosis does not show compositional differences between the two types of synapses.
机译：神经传递涉及停靠的突触小泡在专门的突触前释放部位的钙触发融合。尽管已鉴定出许多参与蛋白，但这些位点的分子组成尚未得到全面表征。在这里，我们报告的程序，以生化分离对接的突触囊泡高度浓缩的部分。该级分基本上不含突触后蛋白和大多数其他细胞器，同时含有最已知的突触小泡和活性区蛋白。还鉴定了许多突触前跨膜蛋白，以及30多种未表征的蛋白，其中许多在进化上是保守的。谷氨酸和GABA特异性对接复合物的定量蛋白质组学比较显示，除神经递质特异性酶和转运蛋白外，在任一馏分中只有很少的蛋白质被选择性富集。我们得出结论，参与囊泡对接和胞吐作用的核心机制没有显示两种突触之间的成分差异。

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