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首页> 外文期刊>Neuron >Subunit configuration of heteromeric cone cyclic nucleotide-gated channels.
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Subunit configuration of heteromeric cone cyclic nucleotide-gated channels.

机译：异聚圆锥环核苷酸门控通道的亚基构型。

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摘要

Cone photoreceptor cyclic nucleotide-gated (CNG) channels are thought to be tetrameric assemblies of CNGB3 (B3) and CNGA3 (A3) subunits. We have used functional and biochemical approaches to investigate the stoichiometry and arrangement of these subunits in recombinant channels. First, tandem dimers of linked subunits were used to constrain the order of CNGB3 and CNGA3 subunits; the properties of channels formed by B3/B3+A3/A3 dimers, or A3/B3+B3/A3 dimers, closely resembled those of channels arising from B3+A3 monomers. Functional markers in B3/B3 (or A3/A3) dimers confirmed that both B3 subunits (and both A3 subunits) gained membership into the pore-forming tetramer and that like subunits were positioned adjacent to each other. Second, chemical crosslinking and co-immunoprecipitation studies using epitope-tagged monomer subunits both demonstrated the presence of two CNGB3 subunits in cone channels. Together, these data support a preferred subunit arrangement for cone CNG channels (B3-B3-A3-A3) that is distinct from the 3A:1B configuration of rod channels.
机译：锥形感光受体环状核苷酸门控（CNG）通道被认为是CNGB3（B3）和CNGA3（A3）亚基的四聚体组装体。我们已经使用功能和生化方法来研究这些化学亚基在重组通道中的化学计量和排列。首先，使用连接的亚基的串联二聚体来约束CNGB3和CNGA3亚基的顺序。由B3 / B3 + A3 / A3二聚体或A3 / B3 + B3 / A3二聚体形成的通道的性质与由B3 + A3单体产生的通道的性质非常相似。 B3 / B3（或A3 / A3）二聚体中的功能标记物证实，两个B3亚基（和两个A3亚基）都已加入形成孔的四聚体中，并且类似的亚基彼此相邻。第二，使用表位标记的单体亚基的化学交联和免疫共沉淀研究均表明在锥通道中存在两个CNGB3亚基。总之，这些数据支持锥形CNG通道（B3-B3-A3-A3）的优选子单元布置，该布置不同于杆状通道的3A：1B配置。

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