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首页> 外文期刊>Neuron >Synaptic vesicles interchange their membrane proteins with a large surface reservoir during recycling.
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Synaptic vesicles interchange their membrane proteins with a large surface reservoir during recycling.

机译：在回收过程中，突触小泡将其膜蛋白与一个较大的表面储库交换。

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During recycling of synaptic vesicles (SVs), the retrieval machinery faces the challenge of recapturing SV proteins in a timely and precise manner. The significant dilution factor that would result from equilibration of vesicle proteins with the much larger cell surface would make recapture by diffusional encounter with the endocytic retrieval machinery unlikely. If SV proteins exchanged with counterparts residing at steady state on the cell surface, the dilution problem would be largely avoided. In this scenario, during electrical activity, endocytosis would be driven by the concentration of a pre-existing pool of SVs residing on the axonal or synaptic surface rather than the heavily diluted postfusion vesicular pool. Using both live cell imaging of endogenous synaptotagmin Ia (sytIa) as well as pHluorin-tagged sytIa and VAMP-2, we show here that synaptic vesicle proteins interchange with a large pool on the cell axonal surface whose concentration is approximately 10-fold lower than that in SVs.
机译：在突触小泡（SVs）的回收过程中，检索设备面临着及时准确地重新捕获SV蛋白的挑战。囊泡蛋白与大得多的细胞表面平衡而产生的显着稀释因子将使通过与内吞回收机制的扩散相遇而重新捕获的可能性很小。如果SV蛋白与稳定存在于细胞表面的对应物交换，将大大避免稀释问题。在这种情况下，在电活动期间，内吞作用将由驻留在轴突或突触表面上的预先存在的SV池的浓度驱动，而不是由大量稀释的融合后囊泡池驱动。使用内源性突触标记素Ia（sytIa）以及pHluorin标记的sytIa和VAMP-2的活细胞成像，我们在这里显示突触小泡蛋白与细胞轴突表面上的一个大池交换，其浓度比其低约10倍。在SV中。

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