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首页> 外文期刊>Neuron >Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating.
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Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating.

机译：突触核蛋白被内啡肽募集以促进突触小泡的脱膜。

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摘要

We describe the isolation and characterization of Drosophila synaptojanin (synj) mutants. synj encodes a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis. We show that Synj is specifically localized to presynaptic terminals and is associated with synaptic vesicles. The electrophysiological and ultrastructural defects observed in synj mutants are strikingly similar to those found in endophilin mutants, and Synj and Endo colocalize and interact biochemically. Moreover, synj; endo double mutant synaptic terminals exhibit properties that are very similar to terminals of each single mutant, and overexpression of Endophilin can partially rescue the functional defects in partial loss-of-function synj mutants. Interestingly, Synj is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synj on newly formed vesicles to promote vesicle uncoating. Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter release when synapses are not extensively challenged.
机译：我们描述了果蝇synaptojanin（synj）突变体的分离和表征。 synj编码参与网格蛋白介导的胞吞作用的磷脂酰肌醇磷酸酶。我们显示，Synj特别是本地化到突触前终端，并与突触囊泡相关联。在synj突变体中观察到的电生理和超微结构缺陷与在endophilin突变体中发现的缺陷极为相似，并且Synj和Endo在化学上共定位并相互作用。而且，synj;内在双突变突触末端显示出与每个单个突变的末端非常相似的特性，Endophilin的过表达可以部分挽救部分功能丧失的synj突变体中的功能缺陷。有趣的是，Synj在没有内啡肽的突触处被错误定位并不稳定，这表明内啡肽在新形成的囊泡上募集并稳定Synj来促进囊泡的脱膜。我们的数据还提供了进一步的证据，表明当突触未受到广泛挑战时，亲吻和奔跑能够维持神经递质的释放。

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