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首页> 外文期刊>Biology and fertility of soils: Cooperating Journal of the International Society of Soil Science >Shifts in the composition and potential functions of soil microbial communities responding to a no-tillage practice and bagasse mulching on a sugarcane plantation
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Shifts in the composition and potential functions of soil microbial communities responding to a no-tillage practice and bagasse mulching on a sugarcane plantation

机译:耕作方式和甘蔗种植地甘蔗渣覆盖对土壤微生物群落组成和潜在功能的影响

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This study examined the effect of conversion from a conventional tillage (CT) to a no-tillage (NT) practice and of bagasse mulching (M) on soil microbial community composition and potential functions, using phospholipid fatty acid (PLFA) analysis and shotgun metagenome sequencing. Our results showed that both the NT and the M treatment increased microbial PLFAs. The shotgun sequencing results suggested that the functional profiles are more resistant to agricultural managements than to community compositions, which supports the hypothesis of the functional redundancy of soil microbial communities. However, some metabolism-related sequences were significantly affected by different treatments. The percentage of sequences related to metabolism of carbohydrates, especially saccharide groups, was significantly higher in the CT soils than in NT and M soils, which may be linked to lower carbon (C) availability in CT soils. Compared with CT, the NT had higher alpha diversity and more sequences related to DNA metabolism, which may be associated with higher nutrient availability. On the other hand, the M treatment decreased the percentages of sequences related to the metabolism of amino acids and derivatives, which may be due to the limited nitrogen (N) because of the high C/N ratio of bagasse. We also observed interaction effects of the NT and M treatments; although both the NT and M treatments increased the relative abundance of Proteobacteria, this variable in NT + M soils was not higher than in each single treatment. Overall, our findings suggest that the microbial communities change their composition and functionality in response to the NT and M treatments, and these shifts have the potential to affect important soil processes that sustain crop productivity, such as C sequestration and major nutrient cycles.
机译:这项研究使用磷脂脂肪酸(PLFA)分析和shot弹枪基因组研究了从传统耕作(CT)转变为免耕(NT)措施以及蔗渣覆盖(M)对土壤微生物群落组成和潜在功能的影响排序。我们的结果表明,NT和M处理均增加了微生物PLFA。 shot弹枪测序结果表明,功能谱对农业管理的抵抗力强于对群落组成的抵抗力,这支持了土壤微生物群落功能冗余的假说。但是,一些与代谢相关的序列受不同处理的影响很大。在CT土壤中,与碳水化合物(尤其是糖基)的代谢相关的序列百分比显着高于NT和M土壤,这可能与CT土壤中较低的碳(C)利用率有关。与CT相比,NT具有更高的α多样性和更多与DNA代谢相关的序列,这可能与更高的养分利用率相关。另一方面,M处理降低了氨基酸和衍生物代谢相关序列的百分比,这可能是由于甘蔗渣的高C / N比导致有限的氮(N)。我们还观察到了NT和M处理的相互作用。尽管NT和M处理均增加了变形杆菌的相对丰度,但NT + M土壤中的这一变量并不高于每个单一处理。总体而言,我们的发现表明,微生物群落响应NT和M处理而改变了它们的组成和功能,这些变化有可能影响维持作物生产力的重要土壤过程,例如固碳和主要养分循环。

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