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Alkaline direct transesterification of different species of Stichococcus for bio-oil production

机译：不同种类链球菌的碱性直接酯交换反应，用于生产生物油

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The cost of bio-oil refining from microalgal biomass can be significantly reduced by combining extraction and transesterification. The characterisation and optimisation of the combined steps have been carried out on strains of Stichococcus bacillaris, focusing on catalyst type and concentration, reaction time and temperature, methanol/biomass ratio, pre-mixing time and water content in the biomass. The bio-oil yield has been referenced as production of fatty acid methyl esters (FAMEs). The maximum yield (similar to 17%) was achieved using dried biomass with alkaline catalyst at 60 degrees C and methanol/biomass weight ratio of 79:1. Alkaline catalyst conditions gave faster reaction rates and higher bio-oil yields than acid catalyst. Yield was also strongly affected by water content in the biomass. A mechanistic interpretation has been proposed to elucidate the effect of the different operating conditions. However, the structural characteristics of the Chlorophyta cell wall can be very different, leading to different bio-oil yields when the same protocol is applied. Therefore, the optimised protocol of direct transesterification for Stichococcus bacillaris strains was tested on other Stichococcus strains and several other Chlorophyta species characterised by a different cell wall structure. It was clearly demonstrated that different results for bio-oil yield were obtained within the same microalgal species and much more within different microalgal genera. (C) 2016 Elsevier B.V. All rights reserved.
机译：通过结合萃取和酯交换反应，可以显着降低从微藻生物质中提炼生物油的成本。已经对细菌链球菌进行了组合步骤的表征和优化，重点是催化剂的类型和浓度，反应时间和温度，甲醇/生物质比，预混合时间和生物质中的水含量。生物油产量已被称为脂肪酸甲酯（FAME）的生产。使用干燥的生物质与碱性催化剂在60摄氏度和甲醇/生物质重量比为79：1时，可获得最大产量（约17％）。碱性催化剂条件比酸性催化剂具有更快的反应速率和更高的生物油产率。产量还受到生物质中水分的强烈影响。已经提出了一种机械解释来阐明不同操作条件的影响。但是，绿藻细胞壁的结构特征可能会非常不同，导致在应用相同方案时生物油产量不同。因此，在其他Stichococcus菌株和其他几种以不同细胞壁结构为特征的Chlorophyta菌种上，测试了细菌Stichococcus bacillaris菌株直接酯交换的优化方案。清楚地表明，在相同的微藻物种中获得了不同的生物油产量结果，而在不同的微藻属中获得了更多的结果。 （C）2016 Elsevier B.V.保留所有权利。

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