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首页> 外文期刊>New biotechnology >Improvement in extracellular protease production by the marine antarctic yeast Rhodotorula mucilaginosa L7
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Improvement in extracellular protease production by the marine antarctic yeast Rhodotorula mucilaginosa L7

机译：海洋南极酵母Rhodotorula mucilaginosa L7改善细胞外蛋白酶的生产

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Microorganisms from extreme and restrictive eco systems, such as the Antarctic continent, are of great interest due to their ability to synthesize products of commercial value. Among these, enzymes from psychrotolerant and psychrophilic microorganisms offer potential economical benefits due to their high activity at low and moderate temperatures. The cold adapted yeast Rhodotorula mucilaginosa L7 was selected out of 97 yeasts isolated from Antarctica as having the highest extracellular proteolytic activity in preliminary tests. The present study was aimed at evaluating the effects of nutrient composition (peptone, rice bran extract, ammonium sulfate, sodium chloride) and physicochemical parameters (temperature and pH) on its proteolytic activity. A 2(6-2) fractional factorial design experiment followed by a central composite design (CCD 2(3)) was performed to optimize the culture conditions and improve the extracellular proteolytic activity. The results indicated that the presence of peptone in the medium was the most influential factor in protease production. Enzymatic activity was enhanced by the interaction between low glucose and peptone concentrations. The optimization of culture conditions with the aid of mathematical modeling enabled a c. 45% increase in proteolytic activity and at the same time reduced the amount of glucose and peptone required for the culture. Thus culture conditions established in this work may be employed in the biotechnological production of this protease. (C) 2016 Elsevier B.V. All rights reserved.
机译：来自极端和限制性生态系统（例如南极大陆）的微生物由于具有合成具有商业价值的产品的能力而备受关注。其中，来自抗精神病和嗜微生物微生物的酶由于其在低温和中温下的高活性而具有潜在的经济效益。在初步测试中，从南极分离的97株酵母中选择了冷适应的酵母Rhodotorula mucilaginosa L7，它们具有最高的细胞外蛋白水解活性。本研究旨在评估营养成分（蛋白p，米糠提取物，硫酸铵，氯化钠）和理化参数（温度和pH）对其蛋白水解活性的影响。进行了2（6-2）分数阶乘设计实验，然后进行了中央复合设计（CCD 2（3）），以优化培养条件并提高细胞外蛋白水解活性。结果表明在培养基中蛋白p的存在是蛋白酶生产中最有影响力的因素。低葡萄糖和蛋白ept浓度之间的相互作用增强了酶活性。培养条件的优化借助数学建模实现了c。蛋白水解活性提高45％，同时减少了培养所需的葡萄糖和蛋白ept的量。因此，在这项工作中建立的培养条件可以用于该蛋白酶的生物技术生产。 （C）2016 Elsevier B.V.保留所有权利。

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