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Growth, lipid extraction and thermal degradation of the microalga Chlorella vulgaris

机译:微藻小球藻的生长,脂质提取和热降解

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The microalga Chlorella vulgaris was cultured in a combined medium obtained by mixing standard Jaworski medium with a solution from the modified Solvay process that contained only NaHCO_3 and NH_4Cl. Cell number, pH and nitrogen content were monitored throughout growth. Lipids were extracted from lyophilised biomass using CHCl_3–MeOH. A combination of grinding, microwave treatment and sonication proved to give the best lipid extract yield. Freeze-dried algal biomass was also utilised for thermal degradation studies. The degradation exhibited three distinct regions – primary cell structure breakage paralleled by evaporation of water, followed by two predominant exothermic degradation processes. The latter were modelled using nth order apparent kinetics. The activation energies of the degradation processes were determined to be 120–126 kJ/mol and 122–132 kJ/mol, respectively. The degradation model may be readily applied to an assortment of thermal algal processes, especially those relating to renewable energy.
机译:将微藻小球藻培养在通过将标准Jaworski培养基与仅包含NaHCO_3和NH_4Cl的改良的Solvay工艺的溶液混合而获得的混合培养基中进行培养。在整个生长过程中监测细胞数量,pH和氮含量。使用CHCl_3-MeOH从冻干的生物质中提取脂质。研磨,微波处理和超声处理的组合被证明可提供最佳的脂质提取物产量。冷冻干燥的藻类生物质也用于热降解研究。降解表现出三个不同的区域–与水蒸发平行的主要细胞结构破坏,随后是两个主要的放热降解过程。后者是使用n阶表观动力学建模的。降解过程的活化能分别确定为120-126 kJ / mol和122-132 kJ / mol。降解模型可以很容易地应用于各种热藻工艺,尤其是与可再生能源有关的工艺。

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