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首页> 外文期刊>New biotechnology >The processive endoglucanase EngZ is active in crystalline cellulose degradation as a cellulosomal subunit of Clostridium cellulovorans
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The processive endoglucanase EngZ is active in crystalline cellulose degradation as a cellulosomal subunit of Clostridium cellulovorans

机译：进行性内切葡聚糖酶EngZ作为纤维素梭菌的纤维素亚单位，在结晶纤维素降解中具有活性。

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摘要

Clostridium cellulovorans produces an efficient enzyme complex for the degradation of lignocellulosic biomass. In our previous study, we detected and identified protein spots that interacted with a fluorescently labeled cohesin biomarker via two-dimensional gel electrophoresis. One novel, putative cellulosomal protein (referred to as endoglucanase Z) contains a catalytic module from the glycosyl hydrolase family (GH9) and demonstrated higher levels of expression than other cellulosomal cellulases in Avicel-containing cultures. Purified EngZ had optimal activity at pH 7.0, 408C, and the major hydrolysis product from the cellooligosaccharides was cellobiose. EngZ’s specific activity toward crystalline cellulose (Avicel and acid-swollen cellulose) was 10–20-fold higher than other cellulosomal cellulase activities. A large percentage of the reducing ends that were produced by this enzyme from acidswollen cellulose were released as soluble sugar. EngZ has the capability of reducing the viscosity of Avicel at an intermediate-level between exo- and endo-typing cellulases, suggesting that it is a processive endoglucanase. In conclusion, EngZ was highly expressed in cellulolytic systems and demonstrated processive endoglucanase activity, suggesting that it plays a major role in the hydrolysis of crystalline cellulose and acts as a cellulosomal enzyme in C. cellulovorans.
机译：纤维梭菌产生用于降解木质纤维素生物质的有效酶复合物。在我们以前的研究中，我们检测和鉴定了通过二维凝胶电泳与荧光标记的黏着素生物标记物相互作用的蛋白质斑点。一种新型的假定纤维素蛋白（称为内切葡聚糖酶Z）包含糖基水解酶家族（GH9）的催化模块，在含Avicel的培养物中比其他纤维素纤维素酶表达水平更高。纯化的EngZ在pH 7.0、408℃下具有最佳活性，纤维寡糖的主要水解产物是纤维二糖。 EngZ对结晶纤维素（微晶纤维素和酸胀性纤维素）的比活性比其他纤维素酶的活性高10-20倍。该酶从酸溶纤维素产生的大部分还原端以可溶性糖的形式释放出来。 EngZ有能力在外切型和内切型纤维素酶之间的中等水平降低Avicel的粘度，这表明它是一种进行性内切葡聚糖酶。总之，EngZ在纤维素分解系统中高表达，并表现出持续的内切葡聚糖酶活性，这表明它在结晶纤维素的水解中起主要作用，并在C. cellulovorans中充当纤维素酶。

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