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首页> 外文期刊>New biotechnology >Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
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Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process

机译：Gordonia alkanivorans菌株1B在二苯并噻吩脱硫过程中的果糖行为

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Biodesulfurization (BDS) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. These microorganisms can remove sulfur from dibenzothiphene (DBT), a model compound, or other polycyclic aromatic used as sulfur source, making BDS an easy and environmental friendly process. Gordonia alkanivorans strain 1B has been described as a desulfurizing bacterium, able to desulfurize DBT to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway, using d-glucose as carbon source. However, both cell growth and desulfurization can be largely affected by the nutrient composition of the growth medium, due to cofactor requirements of many enzymes involved in the BDS biochemical pathway. In this study, the main goal was to investigate the influence of several sugars, as carbon source, on the growth and DBT desulfurization ability of G. alkanivorans strain 1B. The results of desulfurization tests showed that the lowest values for the growth rate (0.025hour~(-1)) and for the overall 2-HBP production rate (1.80μm/hour) by the strain 1B were obtained in glucose grown cultures. When using sucrose, the growth rate increase exhibited by strain 1B led to a higher biomass productivity, which induced a slightly increase in the 2-HBP production rate (1.91μm/hour), conversely in terms of 2-HBP specific production rate (q_(2-HBP)) the value obtained was markedly lower (0.718μmol/g/hour in sucrose versus 1.22μmol/g/hour in glucose). When a mixture of glucose and fructose was used as carbon source, strain 1B reached a value of q_(2-HBP)=1.90μmol/g/hour, close to that in fructose (q_(2-HBP)=2.12μmol/g/hour). The highest values for both cell growth (μ=0.091hour~(-1)) and 2-HPB production (9.29μm/hour) were obtained when strain 1B was desulfurizing DBT in the presence of fructose as the only carbon source, indicating a fructophilic behaviour by this bacterium. This fact is in agreement with the highest value of biomass productivity by strain 1B be in fructose, which resulted in a higher amount cells fulfilling the DBT-desulfurization. The greater number of functional cells conducted to a more effectiveness BDS process by strain 1B, as they attained a q_(2-HBP) about 74% higher than in glucose grown cultures. Moreover, this significant BDS enhancement can better be observed in terms of the overall 2-HBP production rate, which increased over 5-fold, from 1.80μm/hour (in glucose) to 9.29μm/hour (in fructose).
机译：生物脱硫（BDS）的目的是在微生物的帮助下，在温和的操作条件下从化石燃料中去除顽固性硫。这些微生物可以从二苯并噻吩（DBT），模型化合物或其他用作硫源的多环芳烃中去除硫，使BDS成为一种简单且环保的工艺。 Gordonia alkanivorans菌株1B已被描述为一种脱硫细菌，它能够使用d-葡萄糖作为碳源，将DBT脱硫成4S途径的最终产物2-羟基联苯（2-HBP）。然而，由于BDS生化途径中涉及的许多酶的辅因子需求，细胞生长和脱硫都可能受到生长培养基营养成分的很大影响。在这项研究中，主要目标是研究几种糖作为碳源，对链霉菌G.alkanivorans菌株1B的生长和DBT脱硫能力的影响。脱硫试验的结果表明，在葡萄糖生长的培养物中，菌株1B的生长速率（0.025hour〜（-1））和2-HBP的总生产率（1.80μm/ hour）最低。当使用蔗糖时，菌株1B表现出的生长速率增加导致更高的生物量生产率，这导致2-HBP生产率（1.91μm/ hour）略有增加，反之以2-HBP比生产率而言（q_ （2-HBP））得到的值明显更低（蔗糖中为0.718μmol/ g /小时，而葡萄糖中为1.22μmol/ g /小时）。当使用葡萄糖和果糖的混合物作为碳源时，菌株1B的值q_（2-HBP）=1.90μmol/ g / hour，接近果糖中的q_（2-HBP）=2.12μmol/ g /小时）。当菌株1B在果糖作为唯一碳源存在下使DBT脱硫时，获得了最高的细胞生长值（μ= 0.091hour〜（-1））和2-HPB产生值（9.29μm/ hour）。这种细菌的果糖行为。这一事实与果糖中菌株1B产生的最高生物量生产力价值相吻合，这导致产生DBT脱硫的细胞数量更高。菌株1B进行功能更强的BDS处理的功能细胞数量更多，因为它们获得的q_（2-HBP）比葡萄糖培养物中高出约74％。此外，就总的2-HBP生产率而言，这种BDS的显着提高可以更好地观察到，该速率从1.80μm/小时（在葡萄糖中）增加到9.29μm/小时（在果糖中）增加了5倍以上。

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