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Tobacco as platform for a commercial production of cyanophycin

机译:烟草作为商业生产蓝霉素的平台

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Cyanophycin (CP) is a proteinogenic polymer that can be substituted for petroleum in the production of plastic compounds and can also serve as a source of valuable dietary supplements. However, because there is no economically feasible system for large-scale industrial production, its application is limited. In order to develop a low-input system, CP-synthesis was established in the two commercial Nicotiana tabacum (N. tabacum) cultivars 'Badischer Geudertheimer' (BG) and 'Virginia Golta' (VG), by introducing the cyanophycin-synthetase gene from Thermosynecchococcus elongatus BP-1 (CphA(Te)) either via crossbreeding with transgenic N. tabacum cv. Petit Havana SR1 (PH) T2 individual 51-3-2 or by agrobacterium-mediated transformation. Both in F1 hybrids (max. 9.4% CP/DW) and T0 transformants (max. 8.8% CP/DW), a substantial increase in CP content was achieved in leaf tissue, compared to a maximum of 1.7% CP/DW in PH T0 transformants of Huhns et al. (2008). In BG CP, yields were homogenous and there was no substantial difference in the variation of the CP content between primary transformants (T0), clones of T0 individuals, T1 siblings and F1 siblings of hybrids. Therefore, BG meets the requirements for establishing a master seed bank for continuous and reliable CP-production. In addition, it was shown that the polymer is not only stable in planta but also during silage, which simplifies storage of the harvest prior to isolation of CP. (C) 2016 Elsevier B.V. All rights reserved.
机译:蓝藻素(CP)是一种蛋白质生成聚合物,可以在生产塑料化合物时代替石油,也可以用作有价值的膳食补充剂。但是,由于没有在经济上可行的大规模工业生产系统,因此其应用受到限制。为了开发低投入的系统,通过引入蓝霉素合成酶基因,在两个烟草(N. tabacum)品种“ Badischer Geudertheimer”(BG)和“ Virginia Golta”(VG)中建立了CP合成。通过与转基因烟草(N.tabacum)cv。杂交获得来自长嗜热球菌BP-1(CphA(Te))的细菌。小哈瓦那SR1(PH)T2个体为51-3-2或通过农杆菌介导的转化。在F1杂种(最大9.4%CP / DW)和T0转化子(最大8.8%CP / DW)中,叶片组织中CP含量实现了大幅增加,相比之下,PH中最大CP / DW为1.7% Huhns等人的T0转化体。 (2008)。在BG CP中,产量是同质的,初级转化子(T0),T0个体的克隆,T1兄弟姐妹和F1兄弟姐妹之间CP含量的变化没有实质性差异。因此,BG满足建立主种子库以进行连续可靠的CP生产的要求。另外,已表明该聚合物不仅在植物中稳定,而且在青贮饲料中也稳定,这简化了CP分离前收获物的储存。 (C)2016 Elsevier B.V.保留所有权利。

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