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首页> 外文期刊>New biotechnology >Submerged cultivation of Ganoderma lucidum and the effects of its polysaccharides on the production of human cytokines TNF-alpha, IL-12, IFN-gamma, IL-2, IL-4, IL-10 and IL-17
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Submerged cultivation of Ganoderma lucidum and the effects of its polysaccharides on the production of human cytokines TNF-alpha, IL-12, IFN-gamma, IL-2, IL-4, IL-10 and IL-17

机译：灵芝的深层培养及其多糖对人细胞因子TNF-α，IL-12，IFN-γ，IL-2，IL-4，IL-10和IL-17产生的影响

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An original strain of Ganoderma lucidum (W.Curt.:Fr.) Lloyd, MZKI G97 isolated from Slovenian habitats was grown by a submerged liquid substrate cultivation in a laboratory stirred tank reactor. Five fractions of extracellular and cell-wall polysaccharides were obtained by extraction, ethanol precipitation, and purification by ion-exchange, gel and affinity chromatography. The capacity of isolated polysaccharide fractions to induce innate inflammatory cytokines, and to modulate cytokine responses of activated lymphocytes was investigated. Human peripheral blood mononuclear cells (PBMC) were activated in vitro with polysaccharide fractions, in order to induce innate inflammatory cytokines: tumor necrosis factor alpha (TNF-alpha), interleukin (IL) 12 and interferon gamma (IFN-gamma). For the immunomodulation capacity, polysaccharide fractions were cultured with ionomycine and phorbol myristate acetate (IONO + PMA) activated PBMC, and the concentrations of induced IL-2, IL-4, IFN-gamma, IL-10 and IL-17 were measured. The results showed that polysaccharides from G. lucidum induced moderate to high amounts of innate inflammatory cytokines. Fungal cell-wall polysaccharides were stronger innate inflammatory cytokines inducers, while extracellular polysaccharides demonstrated a higher capacity to modulate cytokine responses of IONO + PMA induced production of IL-17. The results indicate that G. lucidum polysaccharides enhance Th1 response with high levels of IFN-gamma and IL-2, and display low to no impact on IL-4 production. A similar pattern was observed at regulatory cytokine IL-10. All of the polysaccharide fractions tested induced IL-17 production at different concentration levels.
机译：通过在实验室搅拌釜反应器中进行浸没液体底物培养来生长从斯洛文尼亚生境分离的原始灵芝（W.Curt.:Fr.)Lloyd MZKI G97菌株。通过提取，乙醇沉淀以及通过离子交换，凝胶和亲和色谱法纯化，获得了五部分的细胞外和细胞壁多糖。研究了分离的多糖级分诱导先天性炎性细胞因子和调节活化淋巴细胞的细胞因子反应的能力。人类外周血单核细胞（PBMC）在体外用多糖级分激活，以诱导先天性炎性细胞因子：肿瘤坏死因子α（TNF-alpha），白介素（IL）12和干扰素γ（IFN-γ）。对于免疫调节能力，用离子霉素和肉豆蔻酸乙酸佛波酯（IONO + PMA）活化的PBMC培养多糖级分，并测量诱导的IL-2，IL-4，IFN-γ，IL-10和IL-17的浓度。结果表明，来自灵芝的多糖诱导中等至大量的先天性炎性细胞因子。真菌细胞壁多糖是更强的先天性炎性细胞因子诱导剂，而细胞外多糖显示出更高的调节IONO + PMA诱导的IL-17产生的细胞因子反应的能力。结果表明，灵芝多糖以高水平的IFN-γ和IL-2增强Th1反应，并且对IL-4产生的影响很小甚至没有影响。在调节性细胞因子IL-10上观察到相似的模式。测试的所有多糖级分均在不同浓度水平下诱导IL-17的产生。

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