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Protection of dried probiotic bacteria from bile using bile adsorbent resins

机译：使用胆汁吸附树脂保护干燥的益生菌免受胆汁污染

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Enteric coated oral tablets or capsules can deliver dried live cells directly into the intestine. Previously, we found that a live attenuated bacterial vaccine acquired sensitivity to intestinal bile when dried, raising the possibility that although gastric acid can be bypassed, significant loss of viability might occur on release from an enteric coated oral formulations. Here we demonstrate that some food-grade lyophilised preparations of Lactobacillus casei and Lactobacillus salivarius also show temporary bile sensitivity that can be rapidly reversed by rehydration. To protect dried bacterial cells from temporary bile sensitivity, we propose using bile acid adsorbing resins, such as cholestyramine, which are bile acid binding agents, historically used to lower cholesterol levels. Vcaps~(?) HPMC capsules alone provided up to 830-fold protection from bile. The inclusion of 50% w/w cholestyramine in Vcaps~(?) HPMC capsules resulted in release of up to 1700-fold more live Lactobacillus casei into simulated intestinal fluid containing 1% bile, when compared to dried cells added directly to bile. We conclude that delivery of dried live probiotic organisms to the intestine may be improved by providing protection from bile by addition of bile adsorbing resins and the use of HPMC capsules.
机译：肠溶衣口服片剂或胶囊剂可以将干燥的活细胞直接递送到肠中。以前，我们发现减毒的活细菌疫苗在干燥后对肠道胆汁具有敏感性，这增加了尽管可以绕过胃酸，但从肠溶衣口服制剂中释放时可能会严重丧失活力的可能性。在这里，我们证明了干酪乳杆菌和唾液乳杆菌的某些食品级冻干制剂也显示出暂时性胆汁敏感性，可以通过补液迅速逆转。为了保护干燥的细菌细胞免受暂时性胆汁敏感性的影响，我们建议使用胆汁酸吸附树脂，例如胆甾醇胺，它们是胆汁酸的结合剂，过去一直用于降低胆固醇水平。仅Vcaps〜（？）HPMC胶囊可提供高达830倍的胆汁保护。与直接添加到胆汁中的干细胞相比，在Vcaps〜（HP）HPMC胶囊中加入50％w / w胆甾醇胺可将多达1700倍的活干酪乳杆菌释放到含1％胆汁的模拟肠液中。我们得出的结论是，通过添加胆汁吸附树脂和使用HPMC胶囊提供对胆汁的保护作用，可以改善干燥活益生菌向肠道的输送。

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