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Succinic acid production from glycerol by Actinobacillus succinogenes using dimethylsulfoxide as electron acceptor

机译:使用二甲基亚砜作为电子受体的琥珀酸放线杆菌由甘油生成甘油的琥珀酸

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摘要

Glycerol, a highly abundant byproduct of the biodiesel industry, constitutes today a cheap feedstock for biobased succinic acid (SA) production. Actinobacillus succinogenes is one of the best SA producers. However, glycerol consumption by this biocatalyst is limited because of a redox imbalance during cell growth. The use of an external electron acceptor may improve the metabolism of SA synthesis by A. succinogenes in glycerol. In this study, the effect of dimethylsulfoxide (DMSO), an electron acceptor, on glycerol consumption and SA production by A. succinogenes under controlled fermentation conditions was investigated. Concentrations of DMSO between 1 and 4% (v/v) greatly promoted glycerol consumption and SA production by A. succinogenes. During fed-batch cultivation, SA concentration reached 49.62. g/L, with a product yield of 0.87. g. SA/g. GLR and a maximum production rate of 2.31. g. SA/L. h, the highest values so far reported in the literature for A. succinogenes using glycerol as carbon source. These results show that using DMSO as external electron acceptor significantly promotes glycerol consumption and succinic acid production by A. succinogenes and may be used as a co-substrate, opening new perspectives for the use of glycerol by this biocatalyst.
机译:甘油是生物柴油工业中高度丰富的副产品,如今已成为生产基于生物的琥珀酸(SA)的廉价原料。琥珀酸放线杆菌是SA最好的生产商之一。然而,由于细胞生长期间氧化还原失衡,该生物催化剂消耗的甘油受到限制。外部电子受体的使用可以改善甘油中的产琥珀酸杆菌引起的SA合成代谢。在这项研究中,研究了在受控发酵条件下,电子受体二甲基亚砜(DMSO)对琥珀酸短杆菌产生甘油的消耗和SA产生的影响。 DMSO的浓度在1%至4%(v / v)之间,可极大地促进琥珀酸假单胞菌消耗甘油并产生SA。在分批补料培养过程中,SA浓度达到49.62。 g / L,产物收率为0.87。 G。 SA / g。 GLR的最大生产率为2.31。 G。 SA / L。 h,文献中报道的使用甘油作为碳源的琥珀酸曲霉的最高值。这些结果表明,使用DMSO作为外部电子受体可显着促进琥珀酸假单胞菌消耗甘油并产生琥珀酸,并且可以用作共底物,从而为该生物催化剂使用甘油开辟了新的前景。

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