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首页> 外文期刊>New biotechnology >Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
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Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates

机译：小麦秸秆木质纤维素水解产物提高聚-3-羟基丁酸酯的生物产量

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Polyhydroxyalkanoates (PHAs) are bioplastics that can replace conventional petroleum-derived products in various applications. One of the major barriers for their widespread introduction in the market is the higher production costs compared with their petrochemical counterparts. In this work, a process was successfully implemented with high productivity based on wheat straw, a cheap and readily available agricultural residue, as raw material. The strain Burkholderia sacchari DSM 17165 which is able to metabolise glucose, xylose and arabinose, the main sugars present in wheat straw hydrolysates (WSHs), was used. Results in shake flask showed that B. sacchari cells accumulated about 70%gpoly(3-hydroxybutyrate)(P(3HB))/g cell dry weight (CDW) with a yield of polymer on sugars (Y_(P/S)) of 0.18g/g when grown on a mixture of commercial C6 and C5 sugars (control), while these values reached about 60%gP(3HB)/g CDW and 0.19g/g, respectively, when WSHs were used as carbon source. In fed-batch cultures carried out in 2L stirred-tank reactors (STRs) on WSH, a maximum polymer concentration of 105g/L was reached after 61hours of cultivation corresponding to an accumulation of 72% of CDW. Polymer yield and productivity were 0.22gP(3HB)/g total sugar consumed and 1.6g/Lhour, respectively. The selected feeding strategy successfully overcame the carbon catabolite repression (CCR) phenomenon observed with sugar mixtures containing hexoses and pentoses. This is the first work describing fed-batch cultivations aiming at PHA production using real lignocellulosic hydrolysates. Additionally, the P(3HB) volumetric productivities attained are by far the highest ever achieved on agricultural waste hydrolysates.
机译：聚羟基链烷酸酯（PHA）是生物塑料，可以在各种应用中替代常规的石油衍生产品。与石化产品相比，它们被广泛引入市场的主要障碍之一是生产成本较高。在这项工作中，成功地实现了以小麦秸秆为原料的高生产率工艺，小麦秸秆是一种廉价且容易获得的农业残留物。使用了能够代谢葡萄糖，木糖和阿拉伯糖（小麦秸秆水解产物（WSHs）中存在的主要糖）的菌株Burkholderia sacchari DSM 17165。摇瓶中的结果表明，B。sacchari细胞积聚约70％的聚（3-羟基丁酸酯）（P（3HB））/ g细胞干重（CDW），而糖的聚合物收率（Y_（P / S））为当使用商品C6和C5糖的混合物（对照）生长时为0.18g / g，而将WSH用作碳源时，这些值分别达到约60％gP（3HB）/ g CDW和0.19g / g。在2L搅拌罐反应器（STRs）上，在WSH上进行的分批补料培养中，培养61小时后，最大聚合物浓度达到105g / L，相当于CDW积累了72％。聚合物的产量和生产率分别为消耗的总糖0.22gP（3HB）/ g和1.6g / Lhour。所选的进食策略成功克服了含己糖和戊糖的糖混合物中观察到的碳分解代谢物抑制（CCR）现象。这是描述使用纯木质纤维素水解物生产PHA的分批补料栽培的第一篇工作。此外，所获得的P（3HB）容积生产率是迄今为止农业废弃物水解产物所能达到的最高生产率。

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