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Rendered-protein hydrolysates for microbial synthesis of cyanophycin biopolymer

机译：提纯蛋白水解物，用于微生物合成氰霉素生物聚合物

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摘要

Cyanophycin is a poly(arginyl-aspartate) biopolymer produced and stored intracellularly by bacteria. Cyanophycin has been proposed as a renewable replacement for petrochemical-based industrial products. An abundant source of amino acids and nitrogen such as in the form of protein hydrolysates is needed for the biosynthesis of cyanophycin. Rendered proteins are largely used as a feed supplement in animal husbandry and aquaculture. New uses would expand the market size of this class of protein coproducts. We prepared and thoroughly characterized the hydrolysates of meat and bone meal, and proceeded to demonstrate for the first time that these hydrolysates could be used in the fermentative production of cyanophycin. Using the enzyme-hydrolyzed meat and bone meal preparation, we obtained crude cyanophycin product at 33-35% level of that produced using the reference casamino acids in both shake-flask and 10-L bioreactor fermentation studies. Polyacrylamide-gel electrophoresis of the cyanophycin under denaturing conditions showed the molecular weight of the isolated polyamide at 24 kDa. Our results open a new avenue for the utilization of rendered protein coproducts to produce the cyanophycin biopolymer.
机译：蓝藻素是一种由细菌产生并储存在细胞内的聚（精氨酸-天冬氨酸）生物聚合物。蓝藻素已被提议作为石化工业产品的可再生替代品。蓝霉素的生物合成需要大量的氨基酸和氮源，例如蛋白质水解产物的形式。提炼的蛋白质在畜牧业和水产养殖业中广泛用作饲料补充。新用途将扩大此类蛋白质副产品的市场规模。我们准备并彻底表征了肉和骨粉的水解产物，并首次证明这些水解产物可用于蓝霉素的发酵生产。使用酶水解的肉类和骨粉制备方法，我们在摇瓶和10 L生物反应器发酵研究中均获得了氰基霉素粗产物的含量，其含量为使用参考酪蛋白氨基酸所产生的粗品的33-35％。在变性条件下，蓝霉素的聚丙烯酰胺凝胶电泳显示分离出的聚酰胺的分子量为24 kDa。我们的结果为利用提炼的蛋白质副产物生产蓝霉素生物聚合物开辟了一条新途径。

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