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首页> 外文期刊>Neuroscience: An International Journal under the Editorial Direction of IBRO >Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons.
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Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons.

机译：培养的大鼠感觉神经元表达的AMPA和红藻氨酸受体的功能相似性和差异。

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摘要

Dorsal root ganglion neurons express functional AMPA and kainate receptors near their central terminals. Activation of these receptors causes a decrease in glutamate release during action potential evoked synaptic transmission. Due to differences in kinetic properties and expression patterns of these two families of glutamate receptors in subpopulations of sensory neurons, AMPA and kainate receptors are expected to function differently. We used embryonic dorsal root ganglion (DRG) neurons maintained in culture to compare functional properties of kainate and AMPA receptors. Most DRG neurons in culture expressed kainate receptors and about half also expressed AMPA receptors. Most AMPA and kainate receptor-expressing DRG neurons were sensitive to capsaicin, suggesting involvement of these glutamate receptors in nociception. When activated by kainate, AMPA receptors were capable of driving a sustained train of action potentials while kainate receptors tended to activate action potential firing more transiently. Glutamate elicited more action potentials and a larger steady-state depolarization in neurons expressing both AMPA and kainate receptors than in neurons expressing only kainate receptors. Adding to their more potent activation properties, AMPA receptors recovered from desensitization much more quickly than kainate receptors. Activation of presynaptic receptors by low concentrations of kainate, but not ATPA, caused a tetrodotoxin-sensitive increase in the frequency of spontaneous EPSCs recorded in dorsal horn neurons. By recording synaptic pairs of DRG and dorsal horn neurons, we found that activation of presynaptic kainate and AMPA receptors decreased evoked glutamate release from terminals of DRG neurons in culture. Our data suggest that the endogenous ligand, glutamate, will cause a different physiological impact when activating these two types of non-NMDA glutamate receptors at central or peripheral nerve endings of sensory neurons.
机译：背根神经节神经元在其中央末端附近表达功能性AMPA和红藻氨酸受体。这些受体的激活引起动作电位诱发的突触传递过程中谷氨酸盐释放的减少。由于感觉神经元亚群中这两个谷氨酸受体家族的动力学性质和表达模式的差异，AMPA和海藻酸盐受体的功能不同。我们使用维持在培养物中的胚胎背根神经节（DRG）神经元来比较海藻酸盐和AMPA受体的功能特性。培养物中大多数DRG神经元表达海藻酸酯受体，约一半还表达AMPA受体。大多数AMPA和表达海藻酸盐受体的DRG神经元对辣椒素敏感，表明这些谷氨酸受体参与了伤害感受。当被海藻酸盐激活时，AMPA受体能够驱动持续的动作电位，而海藻酸盐受体则倾向于更短暂地激活动作电位。与仅表达海藻酸盐受体的神经元相比，谷氨酸在表达AMPA和海藻酸盐受体的神经元中引起更多的动作电位和更大的稳态去极化。除了更有效的激活特性外，AMPA受体从脱敏中恢复的速度比海藻酸酯受体快得多。低浓度的海藻酸盐而非ATPA激活突触前受体，导致背角神经元中记录的自发性EPSC频率对河豚毒素敏感。通过记录DRG和背角神经元的突触对，我们发现，突触前海藻酸盐和AMPA受体的激活减少了培养物中DRG神经元末端诱发的谷氨酸释放。我们的数据表明，内源性配体谷氨酸在激活感觉神经元中枢或末梢神经末梢的这两类非NMDA谷氨酸受体时会引起不同的生理影响。

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