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首页> 外文期刊>Neuroscience: An International Journal under the Editorial Direction of IBRO >Bone marrow stromal cells increase astrocyte survival via upregulation of phosphoinositide 3-kinase/threonine protein kinase and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathways and stimulate astrocyte trophic fa
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Bone marrow stromal cells increase astrocyte survival via upregulation of phosphoinositide 3-kinase/threonine protein kinase and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathways and stimulate astrocyte trophic fa

机译：骨髓基质细胞可通过上调磷酸肌醇3激酶/苏氨酸蛋白激酶和有丝分裂原激活的蛋白激酶激酶/细胞外信号调节的激酶途径来增加星形胶质细胞的存活，并刺激星形胶质细胞营养

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Transplantation of bone marrow stromal cells improves animal neurological functional recovery after stroke. Astrocytes are known to provide structural, trophic and metabolic support for neurons. Thus astrocytes are critical for neural survival during post-ischemia. However, information on the effects of bone marrow stromal cells on astrocytic survival post-ischemia is unavailable. We investigated the influence of rat bone marrow stromal cells on rat astrocytic apoptosis and survival post-ischemia employing an anaerobic chamber. Our data indicate that rat bone marrow stromal cells reduce cell death and apoptosis, and increase the DNA proliferation rate in astrocytes post-ischemia. Mitogen-activated protein kinase kinase/extracellular signal regulated kinase and phosphoinositide 3-kinase/threonine protein kinase pathways are involved in cell survival. Western blot showed that rat bone marrow stromal cells activate these two pathways in astrocytes post-ischemia, and upregulate total extracellular signal regulated kinase 1/2 and threonine protein kinase. Since astrocytes produce various neurotrophic factors, we performed reverse transcription polymerase chain reaction to investigate rat bone marrow stromal cells' effect on astrocyte growth factor gene expression post-ischemia. We observed that brain-derived neurotrophic factor, vascular endothelial growth factor and basic fibroblast growth factor gene expression was enhanced by rat bone marrow stromal cell coculture. These data suggest that bone marrow stromal cells increase astrocytic survival post-ischemic injury. This protective function might involve the activation of mitogen-activated protein kinase kinase/extracellular signal-regulated kinase and phosphoinositide 3-kinase/threonine protein kinase pathways. Upregulation of brain-derived neurotrophic factor, vascular endothelial growth factor and basic fibroblast growth factor may also contribute to astrocyte survival.
机译：骨髓基质细胞的移植改善中风后动物神经功能的恢复。已知星形胶质细胞为神经元提供结构，营养和代谢支持。因此，星形胶质细胞对于缺血后的神经存活至关重要。但是，尚无关于骨髓基质细胞对缺血后星形胶质细胞存活的影响的信息。我们调查了大鼠骨髓基质细胞对大鼠星形细胞凋亡和缺血后存活的影响，采用厌氧室。我们的数据表明，大鼠骨髓基质细胞可减少细胞死亡和凋亡，并增加缺血后星形胶质细胞的DNA增殖速率。丝裂原活化的蛋白激酶激酶/细胞外信号调节激酶和磷酸肌醇3-激酶/苏氨酸蛋白激酶途径参与细胞存活。 Western印迹表明，大鼠骨髓基质细胞在缺血后激活星形胶质细胞中的这两个途径，并上调总的细胞外信号调节激酶1/2和苏氨酸蛋白激酶。由于星形胶质细胞产生各种神经营养因子，我们进行了逆转录聚合酶链反应以研究大鼠骨髓基质细胞对缺血后星形胶质细胞生长因子基因表达的影响。我们观察到，大鼠骨髓基质细胞共培养可增强脑源性神经营养因子，血管内皮生长因子和碱性成纤维细胞生长因子基因的表达。这些数据表明，骨髓基质细胞可增加缺血性损伤后星形胶质细胞的存活。这种保护功能可能涉及激活有丝分裂原激活的蛋白激酶激酶/细胞外信号调节激酶和磷酸肌醇3-激酶/苏氨酸蛋白激酶途径。脑源性神经营养因子，血管内皮生长因子和碱性成纤维细胞生长因子的上调也可能有助于星形胶质细胞的存活。

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