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首页> 外文期刊>Neurosurgery >Alpha(v)beta3 and alpha(v)beta5 integrin expression in glioma periphery.
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Alpha(v)beta3 and alpha(v)beta5 integrin expression in glioma periphery.

机译：胶质瘤外围的Alpha（v）beta3和alpha（v）beta5整合素表达。

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OBJECTIVE: This study analyzed the expression of integrins alpha(v)beta3 and alpha(v)beta5 in glioma tissue and focused on the periphery of high-grade gliomas. METHODS: The analysis was performed with Western blot, immunohistochemistry, and immunofluorescence, by use of two monoclonal antibodies able to recognize the functional integrin heterodimer. The expression of integrin-related ligands and growth factors also was studied. Sections from the tumor periphery were classified as either tumor periphery (light tumor infiltrate or scant visible cells) or peritumor (heavy tumor infiltration). RESULTS: Our data on glioma tissues demonstrated that both integrins were expressed in glioma cells and vasculature and their expression correlated with the histological grade. Alpha(v)beta3 expression was prominent in astrocytic tumors. Both integrins were markers of tumor vasculature, particularly of endothelial proliferation. A high-grade glioma periphery demonstrated a prominent expression of integrin alpha(v)beta3. Cells demonstrating alpha(v)beta3 positivity were identified as tumor astrocytes and endothelial cells by double imaging. The same cells were surrounded by some alpha(v)beta3 ligands and co-localized fibroblast growth factor 2. Matrix metalloproteinase 2 also was found to be co-localized with alpha(v)beta3 in the same cells. Alpha(v)beta3 expression was more relevant in tumor astrocytes. Alpha(v)beta3 integrin and vascular endothelial growth factor expression increased from the periphery to the tumor center. CONCLUSION: Our data support the role of integrins alpha(v)beta3 and alpha(v)beta5 in glioma-associated angiogenesis. In addition, they suggest a role for integrin alpha(v)beta3 in neoangiogenesis and cell migration in high-grade glioma periphery.
机译：目的：本研究分析了整合素α（v）beta3和α（v）beta5在神经胶质瘤组织中的表达，并着眼于高级神经胶质瘤的周围。方法：使用两种能够识别功能整合素异源二聚体的单克隆抗体，通过蛋白质印迹，免疫组织化学和免疫荧光进行分析。还研究了整联蛋白相关配体和生长因子的表达。肿瘤周围的切片分为肿瘤周围（轻度肿瘤浸润或可见细胞很少）或癌旁（重度肿瘤浸润）。结果：我们关于神经胶质瘤组织的数据表明，两种整合素均在神经胶质瘤细胞和脉管系统中表达，并且它们的表达与组织学分级相关。 Alpha（v）beta3表达在星形细胞肿瘤中很明显。两种整合素都是肿瘤脉管系统的标志物，尤其是内皮细胞增殖的标志物。高级神经胶质瘤外围表现出整合素α（v）beta3的突出表达。通过双重成像，表明α（v）β3阳性的细胞被鉴定为肿瘤星形胶质细胞和内皮细胞。相同的细胞被一些alpha（v）beta3配体和共定位的成纤维细胞生长因子2包围。基质金属蛋白酶2也被发现与alpha（v）beta3在同一细胞中共定位。 Alpha（v）beta3表达在肿瘤星形胶质细胞中更相关。 Alpha（v）beta3整合素和血管内皮生长因子的表达从外围到肿瘤中心增加。结论：我们的数据支持整合素α（v）beta3和alpha（v）beta5在神经胶质瘤相关的血管生成中的作用。此外，他们建议整合素α（v）beta3在高级别神经胶质瘤外周血管新生和细胞迁移中的作用。

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