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首页> 外文期刊>Neurosurgery >Expression of endothelial cell angiogenesis receptors in human cerebrovascular malformations.
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Expression of endothelial cell angiogenesis receptors in human cerebrovascular malformations.

机译：内皮细胞血管生成受体在人脑血管畸形中的表达。

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OBJECTIVE: To further understand the role of angiogenic growth factors in the development of cerebral cavernous malformations (CCMs) and arteriovenous malformations (AVMs), we investigated endothelial cell (EC) expression of receptors for vascular endothelial growth factor (VEGF) and angiopoietin systems in patients with surgically resected lesions. METHODS: Paraffin-embedded sections of five AVMs, CCMs, and normal control brain tissue samples were stained immunohistochemically with antibodies to von Willebrand factor and CD31 (to characterize ECs) and angiogenesis growth factor receptors Flt-1 (VEGF-R1), Flk-1 (VEGF-R2), Tie-1, and Tie-2. We counted large and small vessels in each specimen, assessed each specimen's immunoexpression of each antigen, and analyzed differences between CCMs, AVMs, and the normal control brain tissue samples. RESULTS: The ECs of CCMs, AVMs, and normal control brain tissue samples expressed the von Willebrand factor uniformly, but the ECs of CCMs were largely negative for CD31 (P < 0.05). Flk-1, Flt-1, and Tie-2 were not expressed in the control brain tissue samples. The proportion of immunopositive vessels to VEGF receptors Flk-1 and Flt-1 was significantly greater in AVMs and CCMs than in the control brain tissue samples (P < 0.05). Tie-2 in AVMs and CCMs was expressed in a higher percentage of immunopositive vessels than in the control brain tissue samples, but the difference was not statistically significant. Tie-1 was expressed in rare vessels of all lesion types and control brain tissue samples. CONCLUSION: ECs of CCMs do not seem to express CD31 to the same extent that AVMs and normal brain tissue do. AVMs and CCMs show greater expression of VEGF receptors, but not of angiopoietin receptors, than normal brain tissue does.
机译：目的：为进一步了解血管生成生长因子在脑海绵状畸形（CCM）和动静脉畸形（AVM）发展中的作用，我们研究了血管内皮生长因子（VEGF）和血管生成素系统受体的内皮细胞（EC）表达有手术切除病灶的患者。方法：将五个AVM，CCM和正常对照脑组织样品的石蜡包埋切片用von Willebrand因子和CD31抗体（以表征EC）和血管生成生长因子受体Flt-1（VEGF-R1），Flk- 1（VEGF-R2），Tie-1和Tie-2。我们计算了每个标本中的大血管和小血管，评估了每个标本对每种抗原的免疫表达，并分析了CCM，AVM和正常对照脑组织样品之间的差异。结果：CCM，AVM和正常对照脑组织样本的ECs均表达von Willebrand因子，但CCM的CD31大部分呈阴性（P <0.05）。 Flk-1，Flt-1和Tie-2在对照脑组织样本中未表达。在AVM和CCM中，免疫阳性血管相对于VEGF受体Flk-1和Flt-1的比例显着高于对照脑组织样品（P <0.05）。与对照脑组织样品相比，AVM和CCM中的Tie-2在免疫阳性血管中的表达率更高，但差异无统计学意义。 Tie-1在所有病变类型的稀有血管和对照脑组织样本中表达。结论：CCM的ECs表达CD31的程度似乎不及AVM和正常的脑组织。与正常的脑组织相比，AVM和CCM表现出更高的VEGF受体表达，但不表达血管生成素受体。

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