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首页> 外文期刊>Biochemistry >Reconstitution of Okenone into Light Harvesting Complexes from Allochromatium minutissimum
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Reconstitution of Okenone into Light Harvesting Complexes from Allochromatium minutissimum

机译：将烯酮还原成来自别称异色菌的光收集复合物

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摘要

Okenone was reconstituted into light harvesting (LH) complexes of the purple photosynthetic bacterium Allochromatium minutissimum possessing the spirilloxanthin pathway for carotenoid biosynthesis.Suppression of this pathway by diphenylamine,an inhibitor of carotenogenesis,yielded nearly carotenoidless complexes preserving their native spectral properties.Using a previously developed technique,okenone was readily reconstituted into LH1 complex (>90%) whereas its reconstitution into LH2 complex was of low efficacy (10-20%).The absorption band of the reconstituted okenone was shifted to shorter wavelength compared with its position in vivo.This is typical for other reconstituted carotenoids.The reconstitution of okenone was confirmed by Li-DS electrophoresis (in contrast to free okenone the reconstituted okenone migrated with complexes),circular dichroism spectra (reconstituted okenone exhibited optical activity),and fluorescence excitation spectrum (energy transfer from okenone to bacteriochlorophyll was at the control level).
机译：将烯酮重构为具有螺线虫黄质途径的类胡萝卜素生物合成的紫色光合细菌异形异色菌的光收获（LH）复合物。二苯胺可抑制该途径，这是一种类胡萝卜素生成抑制剂，可产生几乎没有类胡萝卜素的复合物，并保留了其天然的光谱特性。随着技术的发展，酮酮很容易被重组为LH1络合物（> 90％），而其重组为LH2络合物的效率很低（10-20％）。与其在体内的位置相比，重组酮的吸收带向较短的波长移动。这对于其他重构类胡萝卜素是典型的。通过Li-DS电泳（与游离的okenone相比，重构的okenone与复合物迁移），圆形二色光谱（重构的okenone表现出光学活性），荧光激发光谱（通过游离的okenone证实了okenone的重构）。能量从酮转移到细菌氯叶绿素处于对照水平）。

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