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A novel intravertebral tumor model in rabbits.

机译：一种新型的椎骨内肿瘤模型。

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OBJECTIVE: Although the majority of human epidural spinal metastases originate in the vertebral body, current animal models of spinal epidural tumors are limited to extraosseous tumor placement. We investigated the onset of paraparesis, radiographic changes (magnetic resonance imaging [MRI] and computed tomographic [CT] scans), and histopathological findings after intraosseous injection of VX2 carcinoma cells into the lower thoracic vertebrae of rabbits. METHODS: New Zealand white rabbits (n = 23) were injected with a 15-mul suspension containing 300,000 VX2 carcinoma cells in the lowest thoracic vertebral body. Lower extremity motor function was assessed daily. For the first 3 animals, MRI scans (T2-weighted and T1-weighted +/- gadolinium) were acquired at postoperative day (POD) 14 and at the onset of paraparesis. Noncontrast CT scans were obtained on POD 7 and at the time of paraparesis. At the onset of paraparesis, the animals ware killed and the spines were dissected. After demineralization, hematoxylin and eosin cross sections were obtained. RESULTS: Before the onset of paraparesis, the CT and MRI scans revealed no gross tumor. At the onset of paraparesis, CT scans demonstrated an osteolytic tumor centered at the junction of the left pedicle and vertebral body, and MRI scans demonstrated epidural tumor arising from the body and compressing the spinal cord. Histopathological examination confirmed carcinoma arising from the body and extending into the canal, with widespread osteolytic activity. By POD 28, 72% of the animals had become paraparetic, and by the termination of the experiment on POD 120, 89% had become paraparetic. CONCLUSION: We established a novel intraosseous intravertebral tumor model in rabbits and characterized it with respect to onset of paraparesis, imaging features, and histopathological findings.
机译：目的：尽管大多数人的硬膜外脊柱转移瘤起源于椎体，但目前的脊柱硬膜外肿瘤动物模型仅限于骨外肿瘤放置。我们对兔下胸椎骨内注射VX2癌细胞后进行了截瘫的发作，影像学改变（磁共振成像[MRI]和计算机断层扫描[CT]扫描）以及组织病理学发现。方法：向新西兰白兔（n = 23）注射最低浓度的15 mul悬浮液，在最低的胸椎椎体中含有300,000个VX2癌细胞。每天评估下肢运动功能。对于前三只动物，在术后第14天和截瘫患者发作时进行MRI扫描（T2加权和T1加权+/- g）。在POD 7上和截瘫时进行了非对比CT扫描。在截瘫患者发作时，将动物杀死并解剖刺。脱矿质后，获得苏木精和曙红的横截面。结果：在截瘫患者发作之前，CT和MRI扫描未发现肉瘤。截瘫时，CT扫描显示溶骨性肿瘤集中在左椎弓根和椎体的交界处，而MRI扫描显示硬膜外肿瘤起源于身体并压迫脊髓。组织病理学检查证实癌发生于机体并延伸到管内，具有广泛的溶骨活性。到POD 28时，有72％的动物变成了寄生虫，而到POD 120上的实验终止，则有89％的动物变成了寄生虫。结论：我们在兔中建立了一种新的骨内椎骨肿瘤模型，并根据轻瘫，影像学特征和组织病理学发现对它进行了表征。

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