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首页> 外文期刊>Neurosurgical focus >Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke
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Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke

机译：颈内动脉间断冷盐水输注作为缺血性中风后神经保护的替代方法

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Object. Intracarotid artery cold saline infusion (ICSI) is an effective method for protecting brain tissue, but its use is limited because of undesirable secondary effects, such as severe decreases in hematocrit levels, as well as its relatively brief duration. In this study, the authors describe and investigate the effects of a novel ICSI pattern (interrupted ICSI) relative to the traditional method (uninterrupted ICSI). Methods. Ischemic strokes were induced in 85 male Sprague-Dawley rats by occluding the middle cerebral artery for 3 hours using an intraluminal filament. Uninterrupted infusion groups received an infusion at 15 ml/hour for 30 minutes continuously. The same infusion speed was used in the interrupted infusion groups, but the whole duration was divided into trisections, and there was a 20-minute interval without infusion between sections. Forty-eight hours after reperfusion, H & E and silver nitrate staining were utilized for morphological assessment. Infarct sizes and brain water contents were determined using H & E staining and the dry-wet weight method, respectively. Levels of neuronspecific enolase (NSE), S100b protein, and matrix metalloproteinase 9 (MMP-9) in the serum were determined using enzyme-linked immunosorbent assay. Neurological deficits were also evaluated. Results. Histology showed that interrupted ICSI did not affect neurons or fibers in rat brains, which suggests that this method is safe for brain tissues with ischemia. The duration of hypothermia induced by interrupted ICSI was longer than that induced via the traditional method, and the decrease in hematocrit levels was less pronounced. There were no differences in infarct size or brain water content between uninterrupted and interrupted ICSI groups, but neuron-specific enolase and matrix metalloproteinase 9 serum levels were more reduced after interrupted ICSI than after the traditional method. Conclusions. Interrupted ICSI is a safe method. Compared with traditional ICSI, the interrupted method has a longer duration of hypothermia and less effect on hematocrit and offers more potentially improved neuroprotection, thereby making it more attractive as an infusion technique in the clinic.
机译：目的。颈内动脉冷盐水输注（ICSI）是一种保护脑组织的有效方法，但是由于不良的继发作用（例如血细胞比容水平的急剧下降以及持续时间相对较短），其使用受到了限制。在这项研究中，作者描述并研究了相对于传统方法（不间断ICSI）的新型ICSI模式（间断ICSI）的影响。方法。通过使用腔内灯丝将大脑中动脉闭塞3小时，在85只Sprague-Dawley雄性大鼠中诱发缺血性中风。不间断输注组以15毫升/小时的速度连续输注30分钟。在中断输注组中使用相同的输注速度，但将整个持续时间分为三等分，并且每节之间有20分钟的间隔不输注。再灌注后48小时，H＆E和硝酸银染色用于形态学评估。分别使用H＆E染色和干湿重法测定梗死面积和脑含水量。使用酶联免疫吸附测定法测定血清中神经元特异性烯醇化酶（NSE），S100b蛋白和基质金属蛋白酶9（MMP-9）的水平。还评估了神经功能缺损。结果。组织学表明，中断的ICSI不会影响大鼠大脑中的神经元或纤维，这表明该方法对于缺血的脑组织是安全的。 ICSI中断引起的体温过低的持续时间比传统方法引起的持续时间长，而血细胞比容水平的降低不那么明显。在不间断的和间断的ICSI组之间，梗塞面积或脑水含量没有差异，但是间断的ICSI后神经元特异性烯醇酶和基质金属蛋白酶9的血清水平较传统方法降低了更多。结论中断ICSI是安全的方法。与传统的ICSI相比，间断法的亚低温持续时间更长，对血细胞比容的影响较小，并且潜在地改善了神经保护作用，因此使其在临床上作为输注技术更具吸引力。

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