Structure and Characterization of the Gene Cluster of the O -Antigen of Escherichia coli 049 Containing 4,6-Dideoxy-4- [(S)-3-hydroxybutanoylamino] -D-glucose

A. V. Perepelov; Quan Wang; S. N. Senchenkova

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Structure and Characterization of the Gene Cluster of the O -Antigen of Escherichia coli 049 Containing 4,6-Dideoxy-4- [(S)-3-hydroxybutanoylamino] -D-glucose

机译：含4,6-二脱氧-4-[（S）-3-羟基丁酰氨基] -D-葡萄糖的大肠杆菌049 O-抗原基因簇的结构与表征

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An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enteropathogenic Escherichia coli 049 and studied by sugar analysis along with one- and two-dimensional ~1H- and ~(13)C-NMR spectroscopy. The following structure of the linear tetrasaccharide repeating unit of the O-polysaccharide was established: →2)-α-D-Quip4N( S3HOBut)-(1→4)-(3-D-GalpNAc-(1→4)-α-L-Rhap-(1→3)-β-D-GlcpNAc6Ac-(1→, where D-Qui4N(S3HOBut) stands for 4,6-dideoxy-4-[(S)-3-hydroxybutanoylamino]-D-glucose and O-acetylation of GlcNAc is partial (～30%). To our knowledge, no N-(3-hydroxybutanoyl) derivative of Qui4N has been hitherto found in bacterial polysaccharides. Gene functions of the O-antigen gene cluster of E. coli O49 were assigned by bioinformatics analysis and found to correspond to the O-polysaccharide structure. Two new genes were revealed and suggested to be responsible for synthesis and transfer of the 3-hydroxybutanoyl group.

机译：通过对肠道致病性大肠杆菌049的脂多糖进行弱酸降解来分离O多糖，并通过糖分析以及一维和二维〜1H-和〜（13）C-NMR光谱进行研究。建立了O-多糖的线性四糖重复单元的以下结构：→2）-α-D-Quip4N（S3HOBut）-（1→4）-（3-D-GalpNAc-（1→4）-α -L-Rhap-（1→3）-β-D-GlcpNAc6Ac-（1→，其中D-Qui4N（S3HOBut）代表4,6-二脱氧-4-[（S）-3-羟基丁酰氨基] -D-葡萄糖和GlcNAc的O-乙酰化是部分的（约30％），据我们所知，到目前为止，在细菌多糖中还没有发现Qui4N的N-（3-羟基丁酰基）衍生物，而E的O抗原基因簇的基因功能。通过生物信息学分析确定了大肠杆菌O49与O-多糖结构相对应，揭示了两个新基因，提示它们负责3-羟基丁酰基的合成和转移。

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《Biochemistry》 |2008年第4期|共5页
作者
A. V. Perepelov; Quan Wang; S. N. Senchenkova;
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正文语种 eng
中图分类生物化学;
关键词
Escherichia coli; lipopolysaccharide; bacterial polysaccharide structure; 3-hydroxybutyrate; 4-amino-4; 6-dideoxy-D-glucose; O-antigen gene cluster;

机译：大肠杆菌;脂多糖;细菌多糖结构;3-羟基丁酸酯;4-氨基-4;6-二脱氧-D-葡萄糖;O-抗原基因簇;

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1. Structure and Characterization of the Gene Cluster of the O -Antigen of Escherichia coli 049 Containing 4,6-Dideoxy-4- [(S)-3-hydroxybutanoylamino] -D-glucose [J] . A. V. Perepelov, Quan Wang, S. N. Senchenkova Biochemistry . 2008,第4期

机译：含4,6-二脱氧-4-[（S）-3-羟基丁酰氨基] -D-葡萄糖的大肠杆菌049 O-抗原基因簇的结构与表征
2. Structure of the O-Antigen and Characterization of the O-Antigen Gene Cluster of Escherichia coli O108 Containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero- D-galacto-non-2-ulosonic (8-Epilegionaminic) Acid [J] . A. V. Perepelov, Bin Liu, S. N. Senchenkova Biochemistry . 2010,第1期

机译：包含5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic（）的大肠杆菌O108的O抗原结构和O抗原基因簇的表征8-Epiegionaminic）酸
3. DNA Sequencing and Identification of Serogroup-Specific Genes in the Escherichia coli O118 O Antigen Gene Cluster and Demonstration of Antigenic Diversity But Only Minor Variation in DNA Sequence of the O Antigen Clusters of E. coli O118 and O151 [J] . Yanhong Liu Pina Fratamico Chitrita Debroy Alyssa C. Bumbaugh John W. Allen. Foodborne Pathogens and Disease . 2008,第4期

机译：大肠杆菌O118 O抗原基因簇中血清群特异性基因的DNA测序和鉴定以及抗原多样性的证明，但O118和O151大肠杆菌O抗原簇的DNA序列仅有微小变化
4. STRUCTURAL AND GENETIC STUDIES ON THE O-ANTIGENS OF ESCHERICHIA ALBERTII, A RECENTLY RECOGNIZED CLOSE RELATIVE OF ESCHERICHIA COLI [C] . Olesya I. Naumenko, Sofya N. Senchenkova, Alexander S. Shashkov International Carbohydrate Symposium . 2018

机译：关于大肠杆菌最近认识到的大肠杆菌的o-antigens的结构和遗传研究
5. Ethylenediaminetetraacetate and nitrilotriacetate degradation by bacterium BNC1: Biochemical characterization of the substrate uptake system and cloning of the entire EDTA-degrading gene cluster in Escherichia coli [D] . Herman, Jacob Paul 2004

机译：细菌BNC1降解乙二胺四乙酸盐和次氮基三乙酸盐：底物吸收系统的生化特性和大肠杆菌中整个EDTA降解基因簇的克隆
6. Molecular Analysis of the O-Antigen Gene Cluster of Escherichia coli O86:B7 and Characterization of the Chain Length Determinant Gene (wzz) [O] . Hongjie Guo, Wen Yi, Jun Shao, 2005

机译：大肠杆菌O86：B7的O抗原基因簇的分子分析和链长决定基因（wzz）的表征
7. Structure and gene cluster of the O-antigen of Escherichia coli O109; chemical and genetic evidences of the presence of l-RhaN3N derivatives in the O-antigens of E. coli O109 and O119 [O] . Andrei V. Perepelov, Zhiwei Ni, Quan Wang, 2010

机译：大肠杆菌O109的O-抗原的结构和基因簇;在大肠杆菌O109和O119的O-抗原中L-RHAN3N衍生物存在的化学和遗传证据

Structure and Characterization of the Gene Cluster of the O -Antigen of Escherichia coli 049 Containing 4,6-Dideoxy-4- [(S)-3-hydroxybutanoylamino] -D-glucose

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