...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Isolation and Crystallization of Heterotrimeric Translation Initiation Factor 2 from Sulfolobus solfataricus
【24h】

Isolation and Crystallization of Heterotrimeric Translation Initiation Factor 2 from Sulfolobus solfataricus

机译：硫枯草菌异三聚体翻译起始因子2的分离和结晶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The structure of the intact heterotrimeric translation initiation factor 2 (e/aIF2) is of great interest due to its key role in the initiator tRNA delivery to the ribosome and in translation initiation regulation in eukaryotes and archaea. We have chosen aIF2 from the hyperthermophilic archaeobacterium Sulfolobus solfataricus (SsoIF2) as an object for crystallization and structural investigations. Genes of the SsoIF2 subunits α, β, and γ were cloned and superexpressed. A method for heterotrimer SsoIF2αβγ purification was elaborated with at least 95% purity. Highly ordered crystals of the full-sized SsoIF2, reflecting X-rays at the resolution up to 2.8 A, were obtained for the first time.
机译：完整的异源三聚翻译起始因子2（e / aIF2）的结构因其在将tRNA传递至核糖体中以及在真核生物和古细菌中的翻译起始调控中起关键作用而引起了人们的极大兴趣。我们从嗜热古生细菌Sulfolobus solfataricus（SsoIF2）中选择了aIF2作为结晶和结构研究的对象。 SsoIF2亚基α，β和γ的基因被克隆并超表达。精心设计了纯度至少为95％的SsoIF2αβγ异源三聚体纯化方法。首次获得了全尺寸SsoIF2的高度有序晶体，该晶体以高达2.8 A的分辨率反射X射线。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号