...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >HI Histone Modulates DNA Hydrolysis with WEN1 and WEN2 Endonucleases from Wheat Coleoptiles
【24h】

HI Histone Modulates DNA Hydrolysis with WEN1 and WEN2 Endonucleases from Wheat Coleoptiles

机译：HI组蛋白调节小麦胚芽鞘中WEN1和WEN2内切核酸酶的DNA水解

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We show that total H1 histone from wheat seedlings or rat liver enhances hydrolysis of A,phage DNA with plant endonucleases WEN1 and WEN2 isolated from wheat coleoptiles.Optimal DNA/protein weight ratio in the hydrolysis reaction is 1:1.The action of fractions I and IV(obtained from total wheat H1 histone by electrophoresis)on DNA hydrolysis with WEN1 and WEN2 enzymes depends on the DNA methylation status.Fraction IV of wheat histone H1 stimulates hydrolysis of unmethylated X phage DNA with WEN1 and WEN2 enzymes.Hydrolysis of methylated A.phage DNA(it contains 5-methylcytosine in Cm~5CWGG sequences and N~6-methyladenine in Gm6ATC sites)with WEN1 is inhibited with fractions I and IV of wheat H1 histone.Fractions II and III of wheat H1 histone do not influence DNA hydrolysis with WEN1 and WEN2.S-Adenosyl-Z-methionine(SAM)stimulates activity of these plant enzymes.But in the presence of H1 histone,SAM does not add to the ability of the enzyme to hydrolyze more DNA compared with that induced with H1 histone itself.Therefore,the stimulating effects of SAM and H1 histone on DNA hydrolysis with plant endonucleases may be similar.It could be suggested that SAM and H1 histone can induce more or less analogous allosteric transformations in the structure of the investigated plant endonucleases.Thus,DNA hydrolysis with plant endonucleases is modulated with total H1 histone.H1 histone fractions affect DNA hydrolysis in a different fashion;they enhance or inhibit hydrolysis depending on the DNA methylation status.We suggest that H1 histone changes site specificity of endonucleases or it might be responsible for formation of new or masking of old sites available for these enzymes due to changes in DNA structure induced in a DNA-histone complex.
机译：结果表明，从小麦胚芽鞘分离的植物内切核酸酶WEN1和WEN2，小麦幼苗或大鼠肝脏中的总H1组蛋白可增强A，噬菌体DNA的水解。水解反应中的最佳DNA /蛋白质重量比为1：1。 WIV1和WEN2酶通过DNA水解产生的IV和IV（通过电泳从小麦H1组蛋白中获得）取决于DNA甲基化状态。小麦组蛋白H1的组分IV刺激WEN1和WEN2酶水解未甲基化的X噬菌体DNA。具有WEN1的噬菌体DNA（在Cm〜5CWGG序列中含有5-甲基胞嘧啶，在Gm6ATC位点中含有N〜6-甲基腺嘌呤）被小麦H1组蛋白的组分I和IV抑制。小麦H1组蛋白的组分II和III不影响DNA S-腺苷-Z-甲硫氨酸（SAM）刺激这些植物酶的活性。但是，在存在H1组蛋白的情况下，与诱导相比，SAM不会增加酶水解更多DNA的能力。因此，SAM和H1组蛋白对植物内切核酸酶对DNA水解的刺激作用可能相似。这表明SAM和H1组蛋白可以在被调查植物的结构中或多或少地诱导类似的变构转化。因此，植物核酸内切酶的DNA水解受总H1组蛋白调控.H1组蛋白组分以不同的方式影响DNA水解;它们根据DNA甲基化状态增强或抑制水解。我们建议H1组蛋白改变核酸内切酶的位点特异性或由于DNA-组蛋白复合物中诱导的DNA结构的变化，它可能负责形成这些酶的新位点或掩盖旧位点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号